Mucor rouxii delta9-desaturase gene is transcriptionally regulated during cell growth and by low temperature

Unsaturated fatty acids are essential lipid components of Mucor rouxii. Gamma-linolenic acid (GLA) is synthesized via the desaturase enzymes: delta9-desaturase catalyzes mono-unsaturated fatty acids that are utilized as substrate for GLA biosynthesis. We cloned and characterized a M. rouxii gene hig...

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Bibliographic Details
Published in:Molecular cell biology research communications 1999-04, Vol.1 (1), p.36
Main Authors: Laoteng, K, Anjard, C, Rachadawong, S, Tanticharoen, M, Maresca, B, Cheevadhanarak, S
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Cell Division
Conserved Sequence
DNA Primers - genetics
Fatty Acids - metabolism
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Fungal
Genes, Fungal
Lipid Metabolism
Molecular Sequence Data
Mucor - enzymology
Mucor - genetics
Mucor - growth & development
RNA, Fungal - genetics
RNA, Fungal - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Homology, Amino Acid
Stearoyl-CoA Desaturase - chemistry
Stearoyl-CoA Desaturase - genetics
Temperature
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Summary:Unsaturated fatty acids are essential lipid components of Mucor rouxii. Gamma-linolenic acid (GLA) is synthesized via the desaturase enzymes: delta9-desaturase catalyzes mono-unsaturated fatty acids that are utilized as substrate for GLA biosynthesis. We cloned and characterized a M. rouxii gene highly homologous to delta9-desaturase genes. This sequence encodes for a protein of 452 amino acids and contains two introns of 60 and 61 nucleotides. Delta9-desaturase of M. rouxii is expressed during cell growth when cells are subjected to temperature shifts. At 30 degrees C, the mRNA level of late log phase is about 6.4-fold higher than that of early log phase. A shift from 30 to 15 degrees C induced transcription of delta9-desaturase gene in both early and late log phases. However, the pattern of increased transcription by cold induction varied depending on growth conditions: transcription of late log phase is higher than that of early log phase. These results indicate that cell growth and low temperature influence the expression of delta9-desaturase gene and fatty acid composition of M. rouxii.
ISSN:1522-4724