Anti-allergic actions of the leaves of Castanea crenata and isolation of an active component responsible for the inhibition of mast cell degranulation

• Published in: Archives of pharmacal research 1999-06, Vol.22 (3), p.320
• Main Authors: Lee, E, Choi, E J, Cheong, H, Kim, Y R, Ryu, S Y, Kim, K M
• Format: Article
• Language: English
• Subjects: Animals; Anti-Allergic Agents - pharmacology; beta-N-Acetylhexosaminidases - secretion; Capillary Permeability - drug effects; Cell Degranulation - drug effects; Dose-Response Relationship, Drug; Histamine - adverse effects; Histamine Release - drug effects; In Vitro Techniques; Male; Mast Cells - metabolism; Passive Cutaneous Anaphylaxis - drug effects; Peritoneum - metabolism; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Quercetin - isolation & purification; Quercetin - pharmacology; Rats; Rats, Sprague-Dawley; Serotonin - adverse effects; Time Factors; Water - chemistry
• ISSN: 0253-6269
• DOI: 10.1007/BF02976372

The anti-allergic actions of the leaves of Castanea crenata (Fagaceae) were studied. The water extract demonstrated potent anti-allergic actions in in vivo and in vitro experiments. The oral or intraperitoneal administration of the extract (100 or 200 mg/kg) caused a significant inhibition of the 48 hr-PCA (up to 90%) and the vascular permeability induced by histamine or serotonin in rats (about 80%). The anaphylactic release of beta-hexosaminidase from RBL-2H3 cells was also significantly inhibited by the extract in a dose-dependent manner with an IC50 value of 230 microg/ml. The activity-guided fractionation of the extract, based on the determination of inhibitory effect upon the release of beta-hexosaminidase, led to the isolation of quercetin as an active principle responsible for the inhibition of degranulation.