Genetic Mapping of Resistance to Bacillus thuringiensis Toxins in Diamondback Moth Using Biphasic Linkage Analysis

Transgenic plants producing environmentally benign Bacillus thuringiensis (Bt) toxins are deployed increasingly for insect control, but their efficacy will be short-lived if pests adapt quickly. The diamondback moth (Plutella xylostella), a worldwide pest of vegetables, is the first insect to evolve...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1999-07, Vol.96 (15), p.8373-8377
Main Authors: Heckel, David G., Gahan, Linda J., Liu, Yong-Biao, Tabashnik, Bruce E.
Format: Article
Language:English
Subjects:
Agricultural biotechnology
Animals
Bacillus thuringiensis
Bacillus thuringiensis - pathogenicity
Bacillus thuringiensis Toxins
Bacteria
Bacterial Proteins - immunology
Bacterial Toxins - immunology
Biological Sciences
Butterflies & moths
Chromosome Mapping
Chromosomes
Cloning, Molecular
Crosses, Genetic
DNA
Endotoxins - immunology
Genes
Genes, Insect
Genetic Linkage
Genetic loci
Genetic Markers
Hemolysin Proteins
Insect genetics
Linkage groups
Molecular Sequence Data
Moths
Moths - genetics
Moths - immunology
Noctuidae
Pesticides
Pests
Plants, Genetically Modified
Plutella xylostella
Toxicology
Toxins
Ultrahigh frequencies
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Summary:Transgenic plants producing environmentally benign Bacillus thuringiensis (Bt) toxins are deployed increasingly for insect control, but their efficacy will be short-lived if pests adapt quickly. The diamondback moth (Plutella xylostella), a worldwide pest of vegetables, is the first insect to evolve resistance to Bt toxins in open-field populations. A recessive autosomal gene confers resistance to at least four Bt toxins and enables survival without adverse effects on transgenic plants. Allelic variants of this gene confer resistance in strains from Hawaii, Pennsylvania, and the Philippines. Here we exploited the biphasic nature of Lepidopteran genetic linkage to map this gene in diamondback moth with 207 amplified fragment length polymorphisms as DNA markers. We also cloned and sequenced an amplified fragment length polymorphism marker for the chromosome containing the Bt resistance gene. The results provide a powerful tool for facilitating progress in understanding, monitoring, and managing resistance to Bt.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.96.15.8373