Development of a New DNA Sequencing Method: 3′-Ester Cleavage Catalyzed by Taq DNA Polymerase

A new 3′-esterified dTTP is incorporated into DNA by Taq DNA polymerase but does not act as a chain terminator. The esterase activity of the polymerase seems to be template dependent and occurs only if the next correct nucleotide is present.

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Bibliographic Details
Published in:Nucleosides & nucleotides 1999-04, Vol.18 (4-5), p.1021-1022
Main Authors: Rasolonjatovo, Isabelle, Sarfati, Simon R.
Format: Article
Language:English
Subjects:
Catalysis
Esters
Hydrolysis
Sequence Analysis, DNA - methods
Taq Polymerase - metabolism
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Description
Summary:A new 3′-esterified dTTP is incorporated into DNA by Taq DNA polymerase but does not act as a chain terminator. The esterase activity of the polymerase seems to be template dependent and occurs only if the next correct nucleotide is present.
ISSN:0732-8311
DOI:10.1080/15257779908041636