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Sub-chronic effect of neem based pesticide (Vepacide) on acetylcholinesterase and ATPases in rat
Acetylcholinesterases (AChE), Na + -K + , Mg 2+ and Ca 2+ -ATPases were monitored in rat brain when treated orally with 80, 160 and 320 mg/kg of Vepacide, an active ingredient from neem seed oil, daily for 90 days. Brain AChE, Na + -K + and Ca 2+ -ATPases were inhibited whereas Mg 2+ -ATPase levels...
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| Published in: | Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes Pesticides, food contaminants, and agricultural wastes, 1999-09, Vol.34 (5), p.873-884 |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c482t-aab6eab14329b36bc0c0536056327a97e71b4f805521edfbd23b4111ab1bf563 |
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| cites | cdi_FETCH-LOGICAL-c482t-aab6eab14329b36bc0c0536056327a97e71b4f805521edfbd23b4111ab1bf563 |
| container_end_page | 884 |
| container_issue | 5 |
| container_start_page | 873 |
| container_title | Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes |
| container_volume | 34 |
| creator | Rahman, M.F Siddiqui, M.K.J Jamil, K |
| description | Acetylcholinesterases (AChE), Na
+
-K
+
, Mg
2+
and Ca
2+
-ATPases were monitored in rat brain when treated orally with 80, 160 and 320 mg/kg of Vepacide, an active ingredient from neem seed oil, daily for 90 days. Brain AChE, Na
+
-K
+
and Ca
2+
-ATPases were inhibited whereas Mg
2+
-ATPase levels were enhanced in both the sexes after 45 and 90 days of treatment. The relative sensitivities of these ATPases to Vepacide indicated that Ca
2+
-ATPase being more sensitive than Na
+
-K
+
-ATPase in both the sexes. The magnitude of Ca
2+
-ATPase inhibited by this compound was higher than that of brain AChE. It appears to be sexual dimorphism in the alterations of brain AChE, Na
+
-K
+
and Mg
2+
-ATPases by Vepacide with females being significant when compared with males. After 28 days of post treatment the alterations observed were approached to those of controls both in male and female rats showing reversal of the toxicity.
These results indicated that the ATPases were potently inhibited by Vepacide and seemed to be its precise target among the enzyme studied. This can be used as biochemical marker of exposure to this neem derived product. |
| doi_str_mv | 10.1080/03601239909373232 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmed_primary_10466107</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17396078</sourcerecordid><originalsourceid>FETCH-LOGICAL-c482t-aab6eab14329b36bc0c0536056327a97e71b4f805521edfbd23b4111ab1bf563</originalsourceid><addsrcrecordid>eNqFkF1rFTEQhoNY7LH6A7zRXIjoxepMsl8Bb0pptVBQ6NHbdZJN7Mpuckz2oOffN4c9olCoVzMwzzO8vIw9Q3iL0MI7kDWgkEqBko0UUjxgK6ykKEoE9ZCt9vciA-Uxe5zSDwBsJdaP2DFCWdcIzYp9u97qwtzE4AfDrXPWzDw47q2duKZke76xaR7M0Fv--qvd0H57w4PnZOy8G81NGAefERszzcn3_HT9Oa-JD55Hmp-wI0djsk8P84StL87XZx-Lq08fLs9OrwpTtmIuiHRtSWMphdKy1gYMVDl-VUvRkGpsg7p0LVSVQNs73QupS0TMinYZOmGvlrebGH5uc55uGpKx40jehm3qsJGqhqbNIC6giSGlaF23icNEcdchdPtWuzutZuf54flWT7b_x1hqzMDLA0DJ0OgieTOkv5xCpVrI2PsFG7wLcaJfIY59N9NuDPGPI--L0fxXv2N18-85my8W01Ho6HvM4JdrAShBKFkrgfIW0Busdg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17396078</pqid></control><display><type>article</type><title>Sub-chronic effect of neem based pesticide (Vepacide) on acetylcholinesterase and ATPases in rat</title><source>Taylor and Francis Science and Technology Collection</source><creator>Rahman, M.F ; Siddiqui, M.K.J ; Jamil, K</creator><creatorcontrib>Rahman, M.F ; Siddiqui, M.K.J ; Jamil, K</creatorcontrib><description>Acetylcholinesterases (AChE), Na
+
-K
+
, Mg
2+
and Ca
2+
-ATPases were monitored in rat brain when treated orally with 80, 160 and 320 mg/kg of Vepacide, an active ingredient from neem seed oil, daily for 90 days. Brain AChE, Na
+
-K
+
and Ca
2+
-ATPases were inhibited whereas Mg
2+
-ATPase levels were enhanced in both the sexes after 45 and 90 days of treatment. The relative sensitivities of these ATPases to Vepacide indicated that Ca
2+
-ATPase being more sensitive than Na
+
-K
+
-ATPase in both the sexes. The magnitude of Ca
2+
-ATPase inhibited by this compound was higher than that of brain AChE. It appears to be sexual dimorphism in the alterations of brain AChE, Na
+
-K
+
and Mg
2+
-ATPases by Vepacide with females being significant when compared with males. After 28 days of post treatment the alterations observed were approached to those of controls both in male and female rats showing reversal of the toxicity.
These results indicated that the ATPases were potently inhibited by Vepacide and seemed to be its precise target among the enzyme studied. This can be used as biochemical marker of exposure to this neem derived product.</description><identifier>ISSN: 0360-1234</identifier><identifier>EISSN: 1532-4109</identifier><identifier>DOI: 10.1080/03601239909373232</identifier><identifier>PMID: 10466107</identifier><identifier>CODEN: JPFCD2</identifier><language>eng</language><publisher>Philadelphia, PA: Taylor & Francis Group</publisher><subject>Acetylcholinesterase ; Acetylcholinesterase - metabolism ; Adenosine Triphosphatases - metabolism ; adenosinetriphosphatase ; Administration, Oral ; and Ca ; Animal, plant and microbial ecology ; Animals ; application rate ; Applied ecology ; ATPases ; Azadirachta indica ; Biological and medical sciences ; brain ; Brain - drug effects ; Brain - enzymology ; Dose-Response Relationship, Drug ; Ecotoxicology, biological effects of pollution ; Effects of pollution and side effects of pesticides on vertebrates ; enzyme activity ; Female ; Fundamental and applied biological sciences. Psychology ; inhibition ; Insecticides - administration & dosage ; Insecticides - toxicity ; Male ; Mammalia ; Medical sciences ; neem ; neem compound ; oral administration ; Pesticides, fertilizers and other agrochemicals toxicology ; rat ; Rats ; Rats, Wistar ; Sex Characteristics ; Toxicology ; Triterpenes - administration & dosage ; Triterpenes - toxicity ; Vepacide</subject><ispartof>Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes, 1999-09, Vol.34 (5), p.873-884</ispartof><rights>Copyright Taylor & Francis Group, LLC 1999</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-aab6eab14329b36bc0c0536056327a97e71b4f805521edfbd23b4111ab1bf563</citedby><cites>FETCH-LOGICAL-c482t-aab6eab14329b36bc0c0536056327a97e71b4f805521edfbd23b4111ab1bf563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1919980$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10466107$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rahman, M.F</creatorcontrib><creatorcontrib>Siddiqui, M.K.J</creatorcontrib><creatorcontrib>Jamil, K</creatorcontrib><title>Sub-chronic effect of neem based pesticide (Vepacide) on acetylcholinesterase and ATPases in rat</title><title>Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes</title><addtitle>J Environ Sci Health B</addtitle><description>Acetylcholinesterases (AChE), Na
+
-K
+
, Mg
2+
and Ca
2+
-ATPases were monitored in rat brain when treated orally with 80, 160 and 320 mg/kg of Vepacide, an active ingredient from neem seed oil, daily for 90 days. Brain AChE, Na
+
-K
+
and Ca
2+
-ATPases were inhibited whereas Mg
2+
-ATPase levels were enhanced in both the sexes after 45 and 90 days of treatment. The relative sensitivities of these ATPases to Vepacide indicated that Ca
2+
-ATPase being more sensitive than Na
+
-K
+
-ATPase in both the sexes. The magnitude of Ca
2+
-ATPase inhibited by this compound was higher than that of brain AChE. It appears to be sexual dimorphism in the alterations of brain AChE, Na
+
-K
+
and Mg
2+
-ATPases by Vepacide with females being significant when compared with males. After 28 days of post treatment the alterations observed were approached to those of controls both in male and female rats showing reversal of the toxicity.
These results indicated that the ATPases were potently inhibited by Vepacide and seemed to be its precise target among the enzyme studied. This can be used as biochemical marker of exposure to this neem derived product.</description><subject>Acetylcholinesterase</subject><subject>Acetylcholinesterase - metabolism</subject><subject>Adenosine Triphosphatases - metabolism</subject><subject>adenosinetriphosphatase</subject><subject>Administration, Oral</subject><subject>and Ca</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>application rate</subject><subject>Applied ecology</subject><subject>ATPases</subject><subject>Azadirachta indica</subject><subject>Biological and medical sciences</subject><subject>brain</subject><subject>Brain - drug effects</subject><subject>Brain - enzymology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Effects of pollution and side effects of pesticides on vertebrates</subject><subject>enzyme activity</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>inhibition</subject><subject>Insecticides - administration & dosage</subject><subject>Insecticides - toxicity</subject><subject>Male</subject><subject>Mammalia</subject><subject>Medical sciences</subject><subject>neem</subject><subject>neem compound</subject><subject>oral administration</subject><subject>Pesticides, fertilizers and other agrochemicals toxicology</subject><subject>rat</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sex Characteristics</subject><subject>Toxicology</subject><subject>Triterpenes - administration & dosage</subject><subject>Triterpenes - toxicity</subject><subject>Vepacide</subject><issn>0360-1234</issn><issn>1532-4109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFkF1rFTEQhoNY7LH6A7zRXIjoxepMsl8Bb0pptVBQ6NHbdZJN7Mpuckz2oOffN4c9olCoVzMwzzO8vIw9Q3iL0MI7kDWgkEqBko0UUjxgK6ykKEoE9ZCt9vciA-Uxe5zSDwBsJdaP2DFCWdcIzYp9u97qwtzE4AfDrXPWzDw47q2duKZke76xaR7M0Fv--qvd0H57w4PnZOy8G81NGAefERszzcn3_HT9Oa-JD55Hmp-wI0djsk8P84StL87XZx-Lq08fLs9OrwpTtmIuiHRtSWMphdKy1gYMVDl-VUvRkGpsg7p0LVSVQNs73QupS0TMinYZOmGvlrebGH5uc55uGpKx40jehm3qsJGqhqbNIC6giSGlaF23icNEcdchdPtWuzutZuf54flWT7b_x1hqzMDLA0DJ0OgieTOkv5xCpVrI2PsFG7wLcaJfIY59N9NuDPGPI--L0fxXv2N18-85my8W01Ho6HvM4JdrAShBKFkrgfIW0Busdg</recordid><startdate>199909</startdate><enddate>199909</enddate><creator>Rahman, M.F</creator><creator>Siddiqui, M.K.J</creator><creator>Jamil, K</creator><general>Taylor & Francis Group</general><general>Taylor & Francis</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>199909</creationdate><title>Sub-chronic effect of neem based pesticide (Vepacide) on acetylcholinesterase and ATPases in rat</title><author>Rahman, M.F ; Siddiqui, M.K.J ; Jamil, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-aab6eab14329b36bc0c0536056327a97e71b4f805521edfbd23b4111ab1bf563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Acetylcholinesterase</topic><topic>Acetylcholinesterase - metabolism</topic><topic>Adenosine Triphosphatases - metabolism</topic><topic>adenosinetriphosphatase</topic><topic>Administration, Oral</topic><topic>and Ca</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>application rate</topic><topic>Applied ecology</topic><topic>ATPases</topic><topic>Azadirachta indica</topic><topic>Biological and medical sciences</topic><topic>brain</topic><topic>Brain - drug effects</topic><topic>Brain - enzymology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Effects of pollution and side effects of pesticides on vertebrates</topic><topic>enzyme activity</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>inhibition</topic><topic>Insecticides - administration & dosage</topic><topic>Insecticides - toxicity</topic><topic>Male</topic><topic>Mammalia</topic><topic>Medical sciences</topic><topic>neem</topic><topic>neem compound</topic><topic>oral administration</topic><topic>Pesticides, fertilizers and other agrochemicals toxicology</topic><topic>rat</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sex Characteristics</topic><topic>Toxicology</topic><topic>Triterpenes - administration & dosage</topic><topic>Triterpenes - toxicity</topic><topic>Vepacide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rahman, M.F</creatorcontrib><creatorcontrib>Siddiqui, M.K.J</creatorcontrib><creatorcontrib>Jamil, K</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rahman, M.F</au><au>Siddiqui, M.K.J</au><au>Jamil, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sub-chronic effect of neem based pesticide (Vepacide) on acetylcholinesterase and ATPases in rat</atitle><jtitle>Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes</jtitle><addtitle>J Environ Sci Health B</addtitle><date>1999-09</date><risdate>1999</risdate><volume>34</volume><issue>5</issue><spage>873</spage><epage>884</epage><pages>873-884</pages><issn>0360-1234</issn><eissn>1532-4109</eissn><coden>JPFCD2</coden><abstract>Acetylcholinesterases (AChE), Na
+
-K
+
, Mg
2+
and Ca
2+
-ATPases were monitored in rat brain when treated orally with 80, 160 and 320 mg/kg of Vepacide, an active ingredient from neem seed oil, daily for 90 days. Brain AChE, Na
+
-K
+
and Ca
2+
-ATPases were inhibited whereas Mg
2+
-ATPase levels were enhanced in both the sexes after 45 and 90 days of treatment. The relative sensitivities of these ATPases to Vepacide indicated that Ca
2+
-ATPase being more sensitive than Na
+
-K
+
-ATPase in both the sexes. The magnitude of Ca
2+
-ATPase inhibited by this compound was higher than that of brain AChE. It appears to be sexual dimorphism in the alterations of brain AChE, Na
+
-K
+
and Mg
2+
-ATPases by Vepacide with females being significant when compared with males. After 28 days of post treatment the alterations observed were approached to those of controls both in male and female rats showing reversal of the toxicity.
These results indicated that the ATPases were potently inhibited by Vepacide and seemed to be its precise target among the enzyme studied. This can be used as biochemical marker of exposure to this neem derived product.</abstract><cop>Philadelphia, PA</cop><pub>Taylor & Francis Group</pub><pmid>10466107</pmid><doi>10.1080/03601239909373232</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0360-1234 |
| ispartof | Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes, 1999-09, Vol.34 (5), p.873-884 |
| issn | 0360-1234 1532-4109 |
| language | eng |
| recordid | cdi_pubmed_primary_10466107 |
| source | Taylor and Francis Science and Technology Collection |
| subjects | Acetylcholinesterase Acetylcholinesterase - metabolism Adenosine Triphosphatases - metabolism adenosinetriphosphatase Administration, Oral and Ca Animal, plant and microbial ecology Animals application rate Applied ecology ATPases Azadirachta indica Biological and medical sciences brain Brain - drug effects Brain - enzymology Dose-Response Relationship, Drug Ecotoxicology, biological effects of pollution Effects of pollution and side effects of pesticides on vertebrates enzyme activity Female Fundamental and applied biological sciences. Psychology inhibition Insecticides - administration & dosage Insecticides - toxicity Male Mammalia Medical sciences neem neem compound oral administration Pesticides, fertilizers and other agrochemicals toxicology rat Rats Rats, Wistar Sex Characteristics Toxicology Triterpenes - administration & dosage Triterpenes - toxicity Vepacide |
| title | Sub-chronic effect of neem based pesticide (Vepacide) on acetylcholinesterase and ATPases in rat |
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