Xeroderma Pigmentosum Cells Contain Low Levels of Photoreactivating Enzyme

Fibroblasts from patients with xeroderma pigmentosum contain low levels of photoreactivating enzyme in comparison to normal cells. Levels vary from 0 (line 1199) to 50 (line 1259) percent of normal. The depressed enzyme levels are not an artifact of low growth rate, age of cell donor, cell culture c...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1975-01, Vol.72 (1), p.103-107
Main Authors: Sutherland, Betsy M., Rice, M., Wagner, E. K.
Format: Article
Language:English
Subjects:
Cell culture techniques
Cell Line
Cell lines
Dimers
DNA Repair
Enzyme activity
Enzymes
Fibroblasts
Fibroblasts - enzymology
Humans
Ichthyosis
Lyases - metabolism
Normal lines
Ultraviolet Rays
Xeroderma pigmentosum
Xeroderma Pigmentosum - enzymology
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Summary:Fibroblasts from patients with xeroderma pigmentosum contain low levels of photoreactivating enzyme in comparison to normal cells. Levels vary from 0 (line 1199) to 50 (line 1259) percent of normal. The depressed enzyme levels are not an artifact of low growth rate, age of cell donor, cell culture conditions, assay conditions, the presence of inhibitors, or mycoplasma contamination. We show that human fibroblasts can monomerize pyrimidine dimers in vivo.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.72.1.103