Quantitative assessment of angiogenesis and osteogenesis after transplantation of bone: Comparison of isograft and allograft bone in mice

We performed a vital microscopic study in mice bearing dorsal skinfold chambers to characterize microvascular perfusion and leukocyte/endothelium interaction and their effects on elongation and mineralization of neonatal isograft and allograft bone. Isograft (C57/BL to C57/BL) and allograft bone (C5...

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Published in:Acta orthopaedica 1999, Vol.70 (4), p.374-380
Main Authors: Leunig, Michael, Demhartner, Thomas J, Sckell, Axel, Fraitzl, Christian R, Gries, Nicola, Sehenk, Robert K, Ganz, Reinhold
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Bone Transplantation - physiology
Immunocompetence
Male
Medical sciences
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Neovascularization, Physiologic
Osteogenesis
Postoperative Period
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Technology. Biomaterials. Equipments
Transplantation, Homologous
Transplantation, Isogeneic
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Summary:We performed a vital microscopic study in mice bearing dorsal skinfold chambers to characterize microvascular perfusion and leukocyte/endothelium interaction and their effects on elongation and mineralization of neonatal isograft and allograft bone. Isograft (C57/BL to C57/BL) and allograft bone (C57/ BL to BALB/C) revascularized simultaneously. However, vascular perfusion and density were lower in allograft bone than in isograft bone. Leukocyte/endothelium interaction was the same in isograft and allograft bones. Revascularization was not detected in allograft bone transplanted to presensitized recipients. Moreover, in preexisting vessels at the trans plantation site, leukocyte/endothelium interaction was altered in allograft bone of presensitized recipients, despite a normal systemic leukocyte count. Femoral growth resulting from thickening of both epiphyses did not differ between experimental groups, however, mineralization occurred in isograft bone only, Isograft bone was histologically intact, allograft bone hypovital and allograft bone in presensitized recipients necrotic 12 days after implantation. Our findings suggest that graft incorporation or rejection is mediated by the microvasculature and that presensi-tizing of recipients accelerates rejection of allograft bone.
ISSN:1745-3674
0001-6470
1745-3682
DOI:10.3109/17453679908997827