Inflammatory action of 8-methoxypsoralen-spermine photoproduct (8-MOP-Spm-P(GFC)) and effects of various drugs on rat paw edema induced by 8-MOP-Spm-P(GFC)

The photoproducts produced by irradiating 8-methoxypsoralen (8-MOP) in the presence of spermine (Spm) were fractionated using gel filtration chromatography (GFC) on a Sephadex G-25 column. As a result, two bands which were characterized by the effects on hyaluronidase activity were obtained. The fir...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 1999-11, Vol.22 (11), p.1202
Main Authors: Shigehara, N, Miyataka, H, Kakegawa, H, Nishiki, M, Matsumoto, H, Isobe, A, Satoh, T
Format: Article
Language:English
Subjects:
Animals
Anti-Inflammatory Agents, Non-Steroidal - pharmacology
Edema - chemically induced
Edema - drug therapy
Erythrocytes - drug effects
Erythrocytes - enzymology
Hemolysis - drug effects
Histamine Antagonists - pharmacology
Hyaluronoglucosaminidase - chemistry
Hydrogen-Ion Concentration
L-Lactate Dehydrogenase - metabolism
Male
Methoxsalen - chemistry
Methoxsalen - toxicity
Molecular Weight
Rats
Rats, Sprague-Dawley
Spermine - chemistry
Spermine - toxicity
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Summary:The photoproducts produced by irradiating 8-methoxypsoralen (8-MOP) in the presence of spermine (Spm) were fractionated using gel filtration chromatography (GFC) on a Sephadex G-25 column. As a result, two bands which were characterized by the effects on hyaluronidase activity were obtained. The first band strongly activated the hyaluronidase, but a second band did not exhibit any effect on the enzyme activity. The first and second bands contained photoproducts with molecular weights (MW)>2700 and MW
ISSN:0918-6158
DOI:10.1248/bpb.22.1202