Nitric oxide release and contractile properties of skeletal muscles from mice deficient in type III NOS

Departments of 1  Medicine, 2  Molecular and Human Genetics, and 3  Anesthesiology, Baylor College of Medicine, Houston, Texas 77030 Skeletal muscle constitutively expresses both the type I (neuronal) and type III (endothelial) isoforms of nitric oxide synthase (NOS). We tested the functional import...

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Published in:American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 2000-01, Vol.278 (1), p.95-R100
Main Authors: Hirschfield, Wulf, Moody, Melanie R, O'Brien, William E, Gregg, Anthony R, Bryan, Robert M., Jr, Reid, Michael B
Format: Article
Language:English
Subjects:
Animals
Chimera
Diaphragm - metabolism
Diaphragm - physiopathology
Heterozygote
Mice
Mice, Inbred C57BL - genetics
Muscle Contraction - physiology
Muscle Fatigue - physiology
Muscle, Skeletal - metabolism
Muscle, Skeletal - physiopathology
Mutation
Nitric Oxide - metabolism
Nitric Oxide Synthase - deficiency
Nitric Oxide Synthase - genetics
Nitric Oxide Synthase Type II
Nitric Oxide Synthase Type III
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Summary:Departments of 1  Medicine, 2  Molecular and Human Genetics, and 3  Anesthesiology, Baylor College of Medicine, Houston, Texas 77030 Skeletal muscle constitutively expresses both the type I (neuronal) and type III (endothelial) isoforms of nitric oxide synthase (NOS). We tested the functional importance of type III NOS using skeletal muscles with similar levels of type III NOS expression (diaphragm and soleus) from wild-type, heterozygous, and type III NOS-deficient littermate mice. Muscles were incubated at 37°C in Krebs-Ringer solution. NO accumulation in the medium was measured by chemiluminescence; force-frequency and fatigue characteristics were measured using direct electrical stimulation. Diaphragm and soleus released NO at similar rates during passive incubation; these rates increased during active contraction. NO release by type III NOS-deficient muscle was not different from that of wild-type muscle under any condition tested. Force-frequency and fatigue characteristics also were unaffected by genotype. Because type III NOS deficiency did not alter function, we conclude that NO effects previously observed in wild-type muscle are likely to be mediated by type I NOS. excitation-contraction coupling; muscle contraction; fatigue; free radicals; diaphragm
ISSN:0363-6119
1522-1490
DOI:10.1152/ajpregu.2000.278.1.r95