Enhancement of Postreplication Repair in Chinese Hamster Cells

Alkaline sedimentation profiles of pulse-labeled DNA from Chinese hamster cells showed that DNA from cells treated with N-acetoxy-acetylaminofluorene or ultraviolet radiation was made in segments smaller than those from untreated cells. Cells treated with a small dose (2.5 μ M) of N-acetoxy-acetylam...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1976-07, Vol.73 (7), p.2396-2400
Main Authors: D'Ambrosio, Steven M., Setlow, R. B.
Format: Article
Language:English
Subjects:
Acetoxyacetylaminofluorene - pharmacology
B lymphocytes
Cell Line
Cell lines
Chinese hamsters
Cycloheximide - pharmacology
Dimers
DNA
DNA - biosynthesis
DNA repair
DNA Repair - drug effects
DNA Repair - radiation effects
DNA Replication
Dose-Response Relationship, Drug
Dose-Response Relationship, Radiation
Irradiation
Molecular Weight
Pulsed radiation
Radiation Effects
Radiotherapy
Ultraviolet Rays
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Summary:Alkaline sedimentation profiles of pulse-labeled DNA from Chinese hamster cells showed that DNA from cells treated with N-acetoxy-acetylaminofluorene or ultraviolet radiation was made in segments smaller than those from untreated cells. Cells treated with a small dose (2.5 μ M) of N-acetoxy-acetylaminofluorene or (2.5 J· m-2) 254-nm radiation, several hours before a larger dose (7-10 μ M) of N-acetoxy-acetylaminofluorene or 5.0 J· m-2of 254-nm radiation, also synthesized small DNA after the second dose. However, the rate at which this small DNA was joined together into parental size was appreciably greater than in absence of the small dose. This enhancement of postreplication repair (as a result of the initial small dose) was not observed when cells were incubated with cycloheximide between the two treatments. The results suggest that N-acetoxy-acetylaminofluorene and ultraviolet-damaged DNA from Chinese hamster cells are repaired by similar postreplicative mechanisms that require de novo protein synthesis for enhancement.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.73.7.2396