Radiation retards muscle differentiation but does not affect osteoblastic differentiation induced by bone morphogenetic protein-2 in C2C12 myoblasts

• Published in: International journal of radiation biology 2000, Vol.76 (3), p.403-411
• Main Author: Ikeda, R. Hachisu, A. Yamaguchi, Y.-H. Gao, T. Okano, S.
• Format: Article
• Language: English
• Subjects: Alkaline Phosphatase - biosynthesis; Animals; Biological and medical sciences; Biological effects of radiation; Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins - metabolism; Bone Morphogenetic Proteins - pharmacology; Cell Differentiation - drug effects; Cell Differentiation - radiation effects; Cell Line; Fundamental and applied biological sciences. Psychology; Humans; Ionizing radiations; Mice; Muscle, Skeletal - cytology; Muscle, Skeletal - drug effects; Muscle, Skeletal - metabolism; Muscle, Skeletal - radiation effects; Myogenin - biosynthesis; Myogenin - genetics; Myosin Heavy Chains - biosynthesis; Myosins - biosynthesis; Myosins - genetics; Osteoblasts - cytology; Osteoblasts - enzymology; Osteocalcin - biosynthesis; Osteocalcin - genetics; Recombinant Proteins - metabolism; Recombinant Proteins - pharmacology; RNA, Messenger - biosynthesis; Space life sciences; Tissues, organs and organisms biophysics; Transforming Growth Factor beta
• ISSN: 0955-3002; 1362-3095
• DOI: 10.1080/095530000138745

Purpose : Effects of radiation on the differentiation of C2C12 myoblasts were studied using cell cultures in which the cells differentiated into either muscle cells or osteoblasts. Materials and methods : A mouse skeletal myoblast cell line, C2C12, was cultured and irradiated at confluence. Muscle differentiation was estimated by counting the number of myosin heavy chain (MHC)-positive multinucleated myotubes. Osteoblast differentiation was analysed by measuring alkaline phosphatase (ALP) activity and osteocalcin production in C2C12 cells treated with recombinant human bone morphogenetic protein-2 (rhBMP-2). Results : Myotube formation in C2C12 cells was inhibited by 2 and 4 Gy irradiation, but recovered to non-irradiated control levels following further culture. In irradiated cultures, the number of MHC-positive mononucleated cells increased significantly. Irradiation did not, however, alter the induction of osteoblastic phenotypes induced by rhBMP-2 in C2C12. Irradiation also did not affect the expression of MyoD mRNA and myogenin mRNA in myogenic differentiation and induction of osteocalcin mRNA in osteoblastic differentiation. Conclusions : During differentiation of C2C12 cells, myotube formation was delayed after irradiation, while induction of osteoblasts with BMP-2 was unaffected.