RNA Polymerase I Transcription Factor Rrn3 Is Functionally Conserved between Yeast and Human

We have cloned a human cDNA that is related to the RNA polymerase I transcription factor Rrn3 of Saccharomyces cerevisiae. The recombinant human protein displays both sequence similarity and immunological crossreactivity to yeast Rrn3 and is capable of rescuing a yeast strain carrying a disruption o...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2000-04, Vol.97 (9), p.4724-4729
Main Authors: Moorefield, Beth, Greene, Elizabeth A., Reeder, Ronald H.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Biological Sciences
Cell growth
Cells
Cloning, Molecular
Complementary DNA
Conserved Sequence
Enzymes
Gene Deletion
Genes
Genetic Complementation Test
Genotype
Humans
Molecular Sequence Data
Open Reading Frames
Pol1 Transcription Initiation Complex Proteins
Proteins
Ribonucleic acid
RNA
RNA Polymerase I - metabolism
RRN3 gene
Rrn3 protein
rRNA
rRNA genes
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae Proteins
Sequence Alignment
Sequence Homology, Amino Acid
Transcription factors
Transcription Factors - chemistry
Transcription Factors - genetics
Transcription Factors - metabolism
Yeast
Yeasts
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Description
Summary:We have cloned a human cDNA that is related to the RNA polymerase I transcription factor Rrn3 of Saccharomyces cerevisiae. The recombinant human protein displays both sequence similarity and immunological crossreactivity to yeast Rrn3 and is capable of rescuing a yeast strain carrying a disruption of the RRN3 gene in vivo. Point mutation of an amino acid that is conserved between the yeast and human proteins compromises the function of each factor, confirming that the observed sequence similarity is functionally significant. Rrn3 is the first RNA polymerase I-specific transcription factor shown to be functionally conserved between yeast and mammals, suggesting that at least one mechanism that regulates ribosomal RNA synthesis is conserved among eukaryotes.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.080063997