Dose-and-time dependent effect of 2,3,7,8-tetrachlorodibenzo-P-dioxin (TCDD) on progesterone secretion by porcine luteal cells cultured in vitro

In the current study, to characterize TCDD action during luteal phase of the ovarian cycle, the direct effect of TCDD was investigated in vitro using a system of monolayer cell culture. Luteal cells isolated from mid-developing corpora lutea were cultured with four different doses of TCDD (0.1, 1.0,...

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Bibliographic Details
Published in:Journal of physiology and pharmacology : an official journal of the Polish Physiological Society 2000-03, Vol.51 (1), p.127
Main Authors: Gregoraszczuk, E L, Wójtowicz, A K, Zabielny, E, Grochowalski, A
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Corpus Luteum - cytology
Corpus Luteum - secretion
Dose-Response Relationship, Drug
Environmental Pollutants - pharmacology
Female
Polychlorinated Dibenzodioxins - pharmacology
Progesterone - antagonists & inhibitors
Progesterone - secretion
Swine
Time Factors
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Summary:In the current study, to characterize TCDD action during luteal phase of the ovarian cycle, the direct effect of TCDD was investigated in vitro using a system of monolayer cell culture. Luteal cells isolated from mid-developing corpora lutea were cultured with four different doses of TCDD (0.1, 1.0, 10.0 and 100 nM). The dose of 0.1nM TCDD had no effect on progesterone (P4) secretion by luteal cells while the doses of 10nM and 100nM in the same, statistically significant manner decreased P4 secretion (p
ISSN:0867-5910