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Quantification of (7S,8R)-Dihydroxy-(9R,10S)-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene Adducts in Human Serum Albumin by Laser-induced Fluorescence: Implications for the in Vivo Metabolism of Benzo[a]pyrene
The ubiquitous environmental carcinogen benzo[ a ]pyrene (BaP) is metabolized in vivo in humans to its ultimate carcinogenic form of 7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[ a ]pyrene (BPDE). Mouse skin tumorigenicity studies indicate that the (7 R ,8 S ,9 S ,10 R ) enantiomer of BPDE, (7...
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| Published in: | Cancer epidemiology, biomarkers & prevention biomarkers & prevention, 2000-07, Vol.9 (7), p.733-739 |
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| container_title | Cancer epidemiology, biomarkers & prevention |
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| creator | ÖZBAL, C. C SKIPPER, P. L YU, M. C LONDON, S. J DASARI, R. R TANNENBAUM, S. R |
| description | The ubiquitous environmental carcinogen benzo[ a ]pyrene
(BaP) is metabolized in vivo in humans to its ultimate
carcinogenic form of
7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[ a ]pyrene
(BPDE). Mouse skin tumorigenicity studies indicate that the
(7 R ,8 S ,9 S ,10 R )
enantiomer of BPDE,
(7 R ,8 S )-dihydroxy-(9 S ,10 R )-epoxy-7,8,9,10-tetrahydrobenzo[ a ]pyrene[
(7 R ,8 S ,9 S ,10 R )-BPDE],
is a potent tumor initiator, whereas the
(7 S ,8 R ,9 R ,10 S )
enantiomer of BPDE,
(7 S ,8 R )-dihydroxy-(9 R ,10 S )-epoxy-7,8,9,10-tetrahydrobenzo[ a ]pyrene[
(7 S ,8 R ,9 R ,10 S )-BPDE],
may act as a tumor promoter. In vitro experiments have
shown that human liver microsomes are capable of metabolizing BaP to
both the
(7 R ,8 S ,9 S ,10 R )
and
(7 S ,8 R ,9 R ,10 S )
enantiomers of BPDE. However, the metabolism of BaP to
(7 S ,8 R ,9 R ,10 S )-BPDE
has not been demonstrated in humans in vivo .
The adducts formed between human serum albumin (HSA) and the
(7 S ,8 R ,9 R ,10 R )
and
(7 R ,8 S ,9 S ,10 R )
enantiomers of BPDE have been described previously.
(7 S ,8 R ,9 R ,10 S )-BPDE
forms a stable adduct at histidine 146 of HSA, whereas
(7 R ,8 S ,9 R ,10 R )-BPDE
forms a relatively unstable ester adduct at aspartate 187 or
glutamate 188 of HSA. Using high-performance liquid
chromatography with laser-induced fluorescence (LIF) detector, we
quantified the level of
(7 S ,8 R ,9 R ,10 S )-BPDE
adducts at histidine 146 in HSA isolated from 63 healthy
males who were population control subjects for an ongoing case-control
study of bladder cancer. By design, roughly half of the participants
were lifelong nonsmokers ( n = 35), whereas the
remaining 28 participants were current smokers of varying intensities.
HP-BPDE adducts were detected in 60 of the 63 samples (95%) by
HPLC-LIF. Adduct levels ranged from undetectable ( |
| format | article |
| fullrecord | <record><control><sourceid>pubmed_pasca</sourceid><recordid>TN_cdi_pubmed_primary_10919745</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10919745</sourcerecordid><originalsourceid>FETCH-LOGICAL-h267t-b3fc7ebac483be8b071824c19e01aa4ea1c255dbca11d9ab254cfb5a1bbf894e3</originalsourceid><addsrcrecordid>eNpVkF9L3EAUxYNU1Np-BZkHKStkIJNkdjK-rbb-gZVSt_VFJNyZ3JiRZBJmkmr8jP1QzborpU_33sOPcw9nJzhgPMmoEJx_mPaIcyrlnO8HH71_iqJISM73gn0WSSZFyg-CPz8GsL0pjYbetJa0JZmJVZjdntCvphoL176MdCZvQxatTih261OEWSgngfbYO3iDFNrX9h4eutGhRbIoikH3nhhLroYGLFmhGxqyqNXQTJoayRI8OmrsxGFBLuqhdeg1Wo2n5Lrp6m0eT8rWkb7CtdWd-d2SG-xBtbXxzTrr2X9_PwW7JdQeP2_nYfDr4tvP8yu6_H55fb5Y0iqei56qpNQCFeg0SxRmKhIsi1PNJEYMIEVgOua8UBoYKySomKe6VByYUmUmU0wOg6ONbzeoBou8c6YBN-bvtU7A8RYAr6EuHVht_D8u5WkcZxP2ZYNV5rF6Ng5zPYHopioQnK5ymYtcJEnyF8zVkog</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Quantification of (7S,8R)-Dihydroxy-(9R,10S)-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene Adducts in Human Serum Albumin by Laser-induced Fluorescence: Implications for the in Vivo Metabolism of Benzo[a]pyrene</title><source>EZB-FREE-00999 freely available EZB journals</source><creator>ÖZBAL, C. C ; SKIPPER, P. L ; YU, M. C ; LONDON, S. J ; DASARI, R. R ; TANNENBAUM, S. R</creator><creatorcontrib>ÖZBAL, C. C ; SKIPPER, P. L ; YU, M. C ; LONDON, S. J ; DASARI, R. R ; TANNENBAUM, S. R</creatorcontrib><description>The ubiquitous environmental carcinogen benzo[ a ]pyrene
(BaP) is metabolized in vivo in humans to its ultimate
carcinogenic form of
7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[ a ]pyrene
(BPDE). Mouse skin tumorigenicity studies indicate that the
(7 R ,8 S ,9 S ,10 R )
enantiomer of BPDE,
(7 R ,8 S )-dihydroxy-(9 S ,10 R )-epoxy-7,8,9,10-tetrahydrobenzo[ a ]pyrene[
(7 R ,8 S ,9 S ,10 R )-BPDE],
is a potent tumor initiator, whereas the
(7 S ,8 R ,9 R ,10 S )
enantiomer of BPDE,
(7 S ,8 R )-dihydroxy-(9 R ,10 S )-epoxy-7,8,9,10-tetrahydrobenzo[ a ]pyrene[
(7 S ,8 R ,9 R ,10 S )-BPDE],
may act as a tumor promoter. In vitro experiments have
shown that human liver microsomes are capable of metabolizing BaP to
both the
(7 R ,8 S ,9 S ,10 R )
and
(7 S ,8 R ,9 R ,10 S )
enantiomers of BPDE. However, the metabolism of BaP to
(7 S ,8 R ,9 R ,10 S )-BPDE
has not been demonstrated in humans in vivo .
The adducts formed between human serum albumin (HSA) and the
(7 S ,8 R ,9 R ,10 R )
and
(7 R ,8 S ,9 S ,10 R )
enantiomers of BPDE have been described previously.
(7 S ,8 R ,9 R ,10 S )-BPDE
forms a stable adduct at histidine 146 of HSA, whereas
(7 R ,8 S ,9 R ,10 R )-BPDE
forms a relatively unstable ester adduct at aspartate 187 or
glutamate 188 of HSA. Using high-performance liquid
chromatography with laser-induced fluorescence (LIF) detector, we
quantified the level of
(7 S ,8 R ,9 R ,10 S )-BPDE
adducts at histidine 146 in HSA isolated from 63 healthy
males who were population control subjects for an ongoing case-control
study of bladder cancer. By design, roughly half of the participants
were lifelong nonsmokers ( n = 35), whereas the
remaining 28 participants were current smokers of varying intensities.
HP-BPDE adducts were detected in 60 of the 63 samples (95%) by
HPLC-LIF. Adduct levels ranged from undetectable (<0.04 fmol/mg HSA)
to 0.77 fmol/mg HSA. The samples had a mean and median
(7 S ,8 R ,9 R ,10 S )-BPDE-HSA
adduct level of 0.22 and 0.16 fmol of adduct/mg albumin, respectively.
Mean adduct levels did not differ between smokers and nonsmokers
( P = 0.72). Occupational exposure to polycyclic
aromatic hydrocarbons was unrelated to adduct level
( P = 0.62). Intake frequencies of two food items
showed statistically significant associations with adduct levels.
Consumption of sweet potatoes was negatively related to adduct level
( P = 0.029), whereas intake of grapefruit juice was
positively related to adduct level ( P = 0.045).
None of the three indices of residential ambient air pollution under
study showed a statistically significant association with adduct
levels.</description><identifier>ISSN: 1055-9965</identifier><identifier>EISSN: 1538-7755</identifier><identifier>PMID: 10919745</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - analysis ; Adult ; Aged ; Benzo(a)pyrene - adverse effects ; Benzo(a)pyrene - metabolism ; Biological and medical sciences ; Carcinogenesis, carcinogens and anticarcinogens ; Case-Control Studies ; Chemical agents ; Chromatography, High Pressure Liquid ; DNA Adducts ; Fluorescence ; Humans ; Male ; Medical sciences ; Middle Aged ; Nephrology. Urinary tract diseases ; Sensitivity and Specificity ; Serum Albumin - genetics ; Serum Albumin - metabolism ; Tumors ; Tumors of the urinary system ; Urinary tract. Prostate gland</subject><ispartof>Cancer epidemiology, biomarkers & prevention, 2000-07, Vol.9 (7), p.733-739</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1454228$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10919745$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ÖZBAL, C. C</creatorcontrib><creatorcontrib>SKIPPER, P. L</creatorcontrib><creatorcontrib>YU, M. C</creatorcontrib><creatorcontrib>LONDON, S. J</creatorcontrib><creatorcontrib>DASARI, R. R</creatorcontrib><creatorcontrib>TANNENBAUM, S. R</creatorcontrib><title>Quantification of (7S,8R)-Dihydroxy-(9R,10S)-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene Adducts in Human Serum Albumin by Laser-induced Fluorescence: Implications for the in Vivo Metabolism of Benzo[a]pyrene</title><title>Cancer epidemiology, biomarkers & prevention</title><addtitle>Cancer Epidemiol Biomarkers Prev</addtitle><description>The ubiquitous environmental carcinogen benzo[ a ]pyrene
(BaP) is metabolized in vivo in humans to its ultimate
carcinogenic form of
7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[ a ]pyrene
(BPDE). Mouse skin tumorigenicity studies indicate that the
(7 R ,8 S ,9 S ,10 R )
enantiomer of BPDE,
(7 R ,8 S )-dihydroxy-(9 S ,10 R )-epoxy-7,8,9,10-tetrahydrobenzo[ a ]pyrene[
(7 R ,8 S ,9 S ,10 R )-BPDE],
is a potent tumor initiator, whereas the
(7 S ,8 R ,9 R ,10 S )
enantiomer of BPDE,
(7 S ,8 R )-dihydroxy-(9 R ,10 S )-epoxy-7,8,9,10-tetrahydrobenzo[ a ]pyrene[
(7 S ,8 R ,9 R ,10 S )-BPDE],
may act as a tumor promoter. In vitro experiments have
shown that human liver microsomes are capable of metabolizing BaP to
both the
(7 R ,8 S ,9 S ,10 R )
and
(7 S ,8 R ,9 R ,10 S )
enantiomers of BPDE. However, the metabolism of BaP to
(7 S ,8 R ,9 R ,10 S )-BPDE
has not been demonstrated in humans in vivo .
The adducts formed between human serum albumin (HSA) and the
(7 S ,8 R ,9 R ,10 R )
and
(7 R ,8 S ,9 S ,10 R )
enantiomers of BPDE have been described previously.
(7 S ,8 R ,9 R ,10 S )-BPDE
forms a stable adduct at histidine 146 of HSA, whereas
(7 R ,8 S ,9 R ,10 R )-BPDE
forms a relatively unstable ester adduct at aspartate 187 or
glutamate 188 of HSA. Using high-performance liquid
chromatography with laser-induced fluorescence (LIF) detector, we
quantified the level of
(7 S ,8 R ,9 R ,10 S )-BPDE
adducts at histidine 146 in HSA isolated from 63 healthy
males who were population control subjects for an ongoing case-control
study of bladder cancer. By design, roughly half of the participants
were lifelong nonsmokers ( n = 35), whereas the
remaining 28 participants were current smokers of varying intensities.
HP-BPDE adducts were detected in 60 of the 63 samples (95%) by
HPLC-LIF. Adduct levels ranged from undetectable (<0.04 fmol/mg HSA)
to 0.77 fmol/mg HSA. The samples had a mean and median
(7 S ,8 R ,9 R ,10 S )-BPDE-HSA
adduct level of 0.22 and 0.16 fmol of adduct/mg albumin, respectively.
Mean adduct levels did not differ between smokers and nonsmokers
( P = 0.72). Occupational exposure to polycyclic
aromatic hydrocarbons was unrelated to adduct level
( P = 0.62). Intake frequencies of two food items
showed statistically significant associations with adduct levels.
Consumption of sweet potatoes was negatively related to adduct level
( P = 0.029), whereas intake of grapefruit juice was
positively related to adduct level ( P = 0.045).
None of the three indices of residential ambient air pollution under
study showed a statistically significant association with adduct
levels.</description><subject>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - analysis</subject><subject>Adult</subject><subject>Aged</subject><subject>Benzo(a)pyrene - adverse effects</subject><subject>Benzo(a)pyrene - metabolism</subject><subject>Biological and medical sciences</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>Case-Control Studies</subject><subject>Chemical agents</subject><subject>Chromatography, High Pressure Liquid</subject><subject>DNA Adducts</subject><subject>Fluorescence</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Sensitivity and Specificity</subject><subject>Serum Albumin - genetics</subject><subject>Serum Albumin - metabolism</subject><subject>Tumors</subject><subject>Tumors of the urinary system</subject><subject>Urinary tract. Prostate gland</subject><issn>1055-9965</issn><issn>1538-7755</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNpVkF9L3EAUxYNU1Np-BZkHKStkIJNkdjK-rbb-gZVSt_VFJNyZ3JiRZBJmkmr8jP1QzborpU_33sOPcw9nJzhgPMmoEJx_mPaIcyrlnO8HH71_iqJISM73gn0WSSZFyg-CPz8GsL0pjYbetJa0JZmJVZjdntCvphoL176MdCZvQxatTih261OEWSgngfbYO3iDFNrX9h4eutGhRbIoikH3nhhLroYGLFmhGxqyqNXQTJoayRI8OmrsxGFBLuqhdeg1Wo2n5Lrp6m0eT8rWkb7CtdWd-d2SG-xBtbXxzTrr2X9_PwW7JdQeP2_nYfDr4tvP8yu6_H55fb5Y0iqei56qpNQCFeg0SxRmKhIsi1PNJEYMIEVgOua8UBoYKySomKe6VByYUmUmU0wOg6ONbzeoBou8c6YBN-bvtU7A8RYAr6EuHVht_D8u5WkcZxP2ZYNV5rF6Ng5zPYHopioQnK5ymYtcJEnyF8zVkog</recordid><startdate>20000701</startdate><enddate>20000701</enddate><creator>ÖZBAL, C. C</creator><creator>SKIPPER, P. L</creator><creator>YU, M. C</creator><creator>LONDON, S. J</creator><creator>DASARI, R. R</creator><creator>TANNENBAUM, S. R</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20000701</creationdate><title>Quantification of (7S,8R)-Dihydroxy-(9R,10S)-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene Adducts in Human Serum Albumin by Laser-induced Fluorescence: Implications for the in Vivo Metabolism of Benzo[a]pyrene</title><author>ÖZBAL, C. C ; SKIPPER, P. L ; YU, M. C ; LONDON, S. J ; DASARI, R. R ; TANNENBAUM, S. R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h267t-b3fc7ebac483be8b071824c19e01aa4ea1c255dbca11d9ab254cfb5a1bbf894e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - analysis</topic><topic>Adult</topic><topic>Aged</topic><topic>Benzo(a)pyrene - adverse effects</topic><topic>Benzo(a)pyrene - metabolism</topic><topic>Biological and medical sciences</topic><topic>Carcinogenesis, carcinogens and anticarcinogens</topic><topic>Case-Control Studies</topic><topic>Chemical agents</topic><topic>Chromatography, High Pressure Liquid</topic><topic>DNA Adducts</topic><topic>Fluorescence</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Sensitivity and Specificity</topic><topic>Serum Albumin - genetics</topic><topic>Serum Albumin - metabolism</topic><topic>Tumors</topic><topic>Tumors of the urinary system</topic><topic>Urinary tract. Prostate gland</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ÖZBAL, C. C</creatorcontrib><creatorcontrib>SKIPPER, P. L</creatorcontrib><creatorcontrib>YU, M. C</creatorcontrib><creatorcontrib>LONDON, S. J</creatorcontrib><creatorcontrib>DASARI, R. R</creatorcontrib><creatorcontrib>TANNENBAUM, S. R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Cancer epidemiology, biomarkers & prevention</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ÖZBAL, C. C</au><au>SKIPPER, P. L</au><au>YU, M. C</au><au>LONDON, S. J</au><au>DASARI, R. R</au><au>TANNENBAUM, S. R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of (7S,8R)-Dihydroxy-(9R,10S)-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene Adducts in Human Serum Albumin by Laser-induced Fluorescence: Implications for the in Vivo Metabolism of Benzo[a]pyrene</atitle><jtitle>Cancer epidemiology, biomarkers & prevention</jtitle><addtitle>Cancer Epidemiol Biomarkers Prev</addtitle><date>2000-07-01</date><risdate>2000</risdate><volume>9</volume><issue>7</issue><spage>733</spage><epage>739</epage><pages>733-739</pages><issn>1055-9965</issn><eissn>1538-7755</eissn><abstract>The ubiquitous environmental carcinogen benzo[ a ]pyrene
(BaP) is metabolized in vivo in humans to its ultimate
carcinogenic form of
7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[ a ]pyrene
(BPDE). Mouse skin tumorigenicity studies indicate that the
(7 R ,8 S ,9 S ,10 R )
enantiomer of BPDE,
(7 R ,8 S )-dihydroxy-(9 S ,10 R )-epoxy-7,8,9,10-tetrahydrobenzo[ a ]pyrene[
(7 R ,8 S ,9 S ,10 R )-BPDE],
is a potent tumor initiator, whereas the
(7 S ,8 R ,9 R ,10 S )
enantiomer of BPDE,
(7 S ,8 R )-dihydroxy-(9 R ,10 S )-epoxy-7,8,9,10-tetrahydrobenzo[ a ]pyrene[
(7 S ,8 R ,9 R ,10 S )-BPDE],
may act as a tumor promoter. In vitro experiments have
shown that human liver microsomes are capable of metabolizing BaP to
both the
(7 R ,8 S ,9 S ,10 R )
and
(7 S ,8 R ,9 R ,10 S )
enantiomers of BPDE. However, the metabolism of BaP to
(7 S ,8 R ,9 R ,10 S )-BPDE
has not been demonstrated in humans in vivo .
The adducts formed between human serum albumin (HSA) and the
(7 S ,8 R ,9 R ,10 R )
and
(7 R ,8 S ,9 S ,10 R )
enantiomers of BPDE have been described previously.
(7 S ,8 R ,9 R ,10 S )-BPDE
forms a stable adduct at histidine 146 of HSA, whereas
(7 R ,8 S ,9 R ,10 R )-BPDE
forms a relatively unstable ester adduct at aspartate 187 or
glutamate 188 of HSA. Using high-performance liquid
chromatography with laser-induced fluorescence (LIF) detector, we
quantified the level of
(7 S ,8 R ,9 R ,10 S )-BPDE
adducts at histidine 146 in HSA isolated from 63 healthy
males who were population control subjects for an ongoing case-control
study of bladder cancer. By design, roughly half of the participants
were lifelong nonsmokers ( n = 35), whereas the
remaining 28 participants were current smokers of varying intensities.
HP-BPDE adducts were detected in 60 of the 63 samples (95%) by
HPLC-LIF. Adduct levels ranged from undetectable (<0.04 fmol/mg HSA)
to 0.77 fmol/mg HSA. The samples had a mean and median
(7 S ,8 R ,9 R ,10 S )-BPDE-HSA
adduct level of 0.22 and 0.16 fmol of adduct/mg albumin, respectively.
Mean adduct levels did not differ between smokers and nonsmokers
( P = 0.72). Occupational exposure to polycyclic
aromatic hydrocarbons was unrelated to adduct level
( P = 0.62). Intake frequencies of two food items
showed statistically significant associations with adduct levels.
Consumption of sweet potatoes was negatively related to adduct level
( P = 0.029), whereas intake of grapefruit juice was
positively related to adduct level ( P = 0.045).
None of the three indices of residential ambient air pollution under
study showed a statistically significant association with adduct
levels.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>10919745</pmid><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1055-9965 |
| ispartof | Cancer epidemiology, biomarkers & prevention, 2000-07, Vol.9 (7), p.733-739 |
| issn | 1055-9965 1538-7755 |
| language | eng |
| recordid | cdi_pubmed_primary_10919745 |
| source | EZB-FREE-00999 freely available EZB journals |
| subjects | 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - analysis Adult Aged Benzo(a)pyrene - adverse effects Benzo(a)pyrene - metabolism Biological and medical sciences Carcinogenesis, carcinogens and anticarcinogens Case-Control Studies Chemical agents Chromatography, High Pressure Liquid DNA Adducts Fluorescence Humans Male Medical sciences Middle Aged Nephrology. Urinary tract diseases Sensitivity and Specificity Serum Albumin - genetics Serum Albumin - metabolism Tumors Tumors of the urinary system Urinary tract. Prostate gland |
| title | Quantification of (7S,8R)-Dihydroxy-(9R,10S)-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene Adducts in Human Serum Albumin by Laser-induced Fluorescence: Implications for the in Vivo Metabolism of Benzo[a]pyrene |
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