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Urinary Excretion of Bowman-Birk Inhibitor in Humans after Soy Consumption as Determined by a Monoclonal Antibody-based Immunoassay
The Bowman-Birk inhibitor (BBI) found in soybeans is a serine protease inhibitor with anticarcinogenic activity. In the present study, an ELISA for BBI was developed with the use of a monoclonal antibody against a reduced form of BBI. This newly developed ELISA method was used to measure the urinary...
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| Published in: | Cancer epidemiology, biomarkers & prevention biomarkers & prevention, 2000-07, Vol.9 (7), p.741-747 |
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| Language: | English |
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| container_end_page | 747 |
| container_issue | 7 |
| container_start_page | 741 |
| container_title | Cancer epidemiology, biomarkers & prevention |
| container_volume | 9 |
| creator | WAN, X. S LU, L.-J. W ANDERSON, K. E WARE, J. H KENNEDY, A. R |
| description | The Bowman-Birk inhibitor (BBI) found in soybeans is a serine protease
inhibitor with anticarcinogenic activity. In the present study, an
ELISA for BBI was developed with the use of a monoclonal antibody
against a reduced form of BBI. This newly developed ELISA method was
used to measure the urinary levels of BBI metabolites in nine human
subjects after consumption of 36-oz or 60-oz soymilk (containing 105 or
175 mg of BBI) at two time points 36 h apart. The results
demonstrate that urinary BBI excretion rates peaked within 6 h and
decreased to baseline levels within 12–24 h after soymilk ingestion.
The changes in BBI:creatinine ratios in urine closely paralleled the
changes in urinary BBI excretion rates after soymilk consumption. These
data suggest that BBI ingested p.o. is absorbed and could be
bioavailable for cancer chemoprevention in other organs in addition to
those in the gastrointestinal tract. |
| format | article |
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inhibitor with anticarcinogenic activity. In the present study, an
ELISA for BBI was developed with the use of a monoclonal antibody
against a reduced form of BBI. This newly developed ELISA method was
used to measure the urinary levels of BBI metabolites in nine human
subjects after consumption of 36-oz or 60-oz soymilk (containing 105 or
175 mg of BBI) at two time points 36 h apart. The results
demonstrate that urinary BBI excretion rates peaked within 6 h and
decreased to baseline levels within 12–24 h after soymilk ingestion.
The changes in BBI:creatinine ratios in urine closely paralleled the
changes in urinary BBI excretion rates after soymilk consumption. These
data suggest that BBI ingested p.o. is absorbed and could be
bioavailable for cancer chemoprevention in other organs in addition to
those in the gastrointestinal tract.</description><identifier>ISSN: 1055-9965</identifier><identifier>EISSN: 1538-7755</identifier><identifier>PMID: 10919746</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Adult ; Antibodies, Monoclonal ; Biological and medical sciences ; Biological Availability ; Carcinogenesis, carcinogens and anticarcinogens ; Creatinine - urine ; Diet ; Enzyme-Linked Immunosorbent Assay ; Female ; Foods and miscellaneous ; Glycine max - chemistry ; Humans ; Male ; Medical sciences ; Trypsin Inhibitor, Bowman-Birk Soybean - pharmacokinetics ; Trypsin Inhibitor, Bowman-Birk Soybean - urine ; Trypsin Inhibitors - pharmacokinetics ; Trypsin Inhibitors - urine ; Tumors</subject><ispartof>Cancer epidemiology, biomarkers & prevention, 2000-07, Vol.9 (7), p.741-747</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1454556$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10919746$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WAN, X. S</creatorcontrib><creatorcontrib>LU, L.-J. W</creatorcontrib><creatorcontrib>ANDERSON, K. E</creatorcontrib><creatorcontrib>WARE, J. H</creatorcontrib><creatorcontrib>KENNEDY, A. R</creatorcontrib><title>Urinary Excretion of Bowman-Birk Inhibitor in Humans after Soy Consumption as Determined by a Monoclonal Antibody-based Immunoassay</title><title>Cancer epidemiology, biomarkers & prevention</title><addtitle>Cancer Epidemiol Biomarkers Prev</addtitle><description>The Bowman-Birk inhibitor (BBI) found in soybeans is a serine protease
inhibitor with anticarcinogenic activity. In the present study, an
ELISA for BBI was developed with the use of a monoclonal antibody
against a reduced form of BBI. This newly developed ELISA method was
used to measure the urinary levels of BBI metabolites in nine human
subjects after consumption of 36-oz or 60-oz soymilk (containing 105 or
175 mg of BBI) at two time points 36 h apart. The results
demonstrate that urinary BBI excretion rates peaked within 6 h and
decreased to baseline levels within 12–24 h after soymilk ingestion.
The changes in BBI:creatinine ratios in urine closely paralleled the
changes in urinary BBI excretion rates after soymilk consumption. These
data suggest that BBI ingested p.o. is absorbed and could be
bioavailable for cancer chemoprevention in other organs in addition to
those in the gastrointestinal tract.</description><subject>Adult</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Biological Availability</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>Creatinine - urine</subject><subject>Diet</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Foods and miscellaneous</subject><subject>Glycine max - chemistry</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Trypsin Inhibitor, Bowman-Birk Soybean - pharmacokinetics</subject><subject>Trypsin Inhibitor, Bowman-Birk Soybean - urine</subject><subject>Trypsin Inhibitors - pharmacokinetics</subject><subject>Trypsin Inhibitors - urine</subject><subject>Tumors</subject><issn>1055-9965</issn><issn>1538-7755</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNpF0EtPwzAMAOAKgdgY_AWUA-JWKV3zaI7beGzSEAfYuXIepYE1mZJWo2f-OIUNcbJlf7ZknyTjjOZFyjmlp0OOKU2FYHSUXMT4jjHmgtLzZJRhkQlO2Dj52gTrIPTo_lMF01rvkK_Q3O8bcOnchg-0crWVtvUBWYeW3VCPCKrWBPTie7TwLnbN7ncQIrozQ6OxzmgkewToyTuvtt7BFs1ca6XXfSohDu1V03TOQ4zQXyZnFWyjuTrGSbJ5uH9dLNP18-NqMVun9ZTxNiWZVhiMEVoTLotCYgYgmZSgcZHrijE91bgSQuYVaElyoozJmAIy1VIDzSfJ9WHvrpON0eUu2GY4vfz7xgBujgCigm0VwCkb_x2hhNIfdntgtX2r9zaYUg3QhGCigaDqUpS85CTLvwEMuXrt</recordid><startdate>20000701</startdate><enddate>20000701</enddate><creator>WAN, X. S</creator><creator>LU, L.-J. W</creator><creator>ANDERSON, K. E</creator><creator>WARE, J. H</creator><creator>KENNEDY, A. R</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20000701</creationdate><title>Urinary Excretion of Bowman-Birk Inhibitor in Humans after Soy Consumption as Determined by a Monoclonal Antibody-based Immunoassay</title><author>WAN, X. S ; LU, L.-J. W ; ANDERSON, K. E ; WARE, J. H ; KENNEDY, A. R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h267t-41dc0aee9dd47b88b06aab6bbad083df66d2d0f99b3fadb434cee16ca42dbda53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Biological Availability</topic><topic>Carcinogenesis, carcinogens and anticarcinogens</topic><topic>Creatinine - urine</topic><topic>Diet</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Foods and miscellaneous</topic><topic>Glycine max - chemistry</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Trypsin Inhibitor, Bowman-Birk Soybean - pharmacokinetics</topic><topic>Trypsin Inhibitor, Bowman-Birk Soybean - urine</topic><topic>Trypsin Inhibitors - pharmacokinetics</topic><topic>Trypsin Inhibitors - urine</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WAN, X. S</creatorcontrib><creatorcontrib>LU, L.-J. W</creatorcontrib><creatorcontrib>ANDERSON, K. E</creatorcontrib><creatorcontrib>WARE, J. H</creatorcontrib><creatorcontrib>KENNEDY, A. R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Cancer epidemiology, biomarkers & prevention</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WAN, X. S</au><au>LU, L.-J. W</au><au>ANDERSON, K. E</au><au>WARE, J. H</au><au>KENNEDY, A. R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Urinary Excretion of Bowman-Birk Inhibitor in Humans after Soy Consumption as Determined by a Monoclonal Antibody-based Immunoassay</atitle><jtitle>Cancer epidemiology, biomarkers & prevention</jtitle><addtitle>Cancer Epidemiol Biomarkers Prev</addtitle><date>2000-07-01</date><risdate>2000</risdate><volume>9</volume><issue>7</issue><spage>741</spage><epage>747</epage><pages>741-747</pages><issn>1055-9965</issn><eissn>1538-7755</eissn><abstract>The Bowman-Birk inhibitor (BBI) found in soybeans is a serine protease
inhibitor with anticarcinogenic activity. In the present study, an
ELISA for BBI was developed with the use of a monoclonal antibody
against a reduced form of BBI. This newly developed ELISA method was
used to measure the urinary levels of BBI metabolites in nine human
subjects after consumption of 36-oz or 60-oz soymilk (containing 105 or
175 mg of BBI) at two time points 36 h apart. The results
demonstrate that urinary BBI excretion rates peaked within 6 h and
decreased to baseline levels within 12–24 h after soymilk ingestion.
The changes in BBI:creatinine ratios in urine closely paralleled the
changes in urinary BBI excretion rates after soymilk consumption. These
data suggest that BBI ingested p.o. is absorbed and could be
bioavailable for cancer chemoprevention in other organs in addition to
those in the gastrointestinal tract.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>10919746</pmid><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 1055-9965 |
| ispartof | Cancer epidemiology, biomarkers & prevention, 2000-07, Vol.9 (7), p.741-747 |
| issn | 1055-9965 1538-7755 |
| language | eng |
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| source | EZB Electronic Journals Library |
| subjects | Adult Antibodies, Monoclonal Biological and medical sciences Biological Availability Carcinogenesis, carcinogens and anticarcinogens Creatinine - urine Diet Enzyme-Linked Immunosorbent Assay Female Foods and miscellaneous Glycine max - chemistry Humans Male Medical sciences Trypsin Inhibitor, Bowman-Birk Soybean - pharmacokinetics Trypsin Inhibitor, Bowman-Birk Soybean - urine Trypsin Inhibitors - pharmacokinetics Trypsin Inhibitors - urine Tumors |
| title | Urinary Excretion of Bowman-Birk Inhibitor in Humans after Soy Consumption as Determined by a Monoclonal Antibody-based Immunoassay |
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