Effect of combined treatment with perindoprilat and low-power red light laser irradiation on human erythrocyte membrane fluidity, membrane potential and acetylcholinesterase activity

Erythrocyte membrane fluidity, membrane potential and acetylcholinesterase activity were estimated after in vitro combined treatment of human erythrocytes with perindoprilat and low-power red light irradiation. Membrane fluidity was determined using fluorescent labels spectroscopy; membrane potentia...

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Bibliographic Details
Published in:Scandinavian journal of clinical and laboratory investigation 2000, Vol.60 (5), p.395-402
Main Authors: Piasecka, A., Leyko, W., Krajewska, E., Bryszewska, M.
Format: Article
Language:English
Subjects:
Acetylcholinesterase - metabolism
Angiotensin-Converting Enzyme Inhibitors - pharmacology
Biological and medical sciences
Cardiovascular system
Color
Diphenylhexatriene - analogs & derivatives
Diphenylhexatriene - metabolism
Diseases of the cardiovascular system
Erythrocytes - drug effects
Erythrocytes - enzymology
Erythrocytes - physiology
Erythrocytes - radiation effects
Humans
Indoles - pharmacology
Kinetics
Lasers
Medical sciences
Membrane Fluidity - drug effects
Membrane Fluidity - radiation effects
Membrane Potentials - drug effects
Membrane Potentials - radiation effects
Miscellaneous
Pharmacology. Drug treatments
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Spectrometry, Fluorescence
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Summary:Erythrocyte membrane fluidity, membrane potential and acetylcholinesterase activity were estimated after in vitro combined treatment of human erythrocytes with perindoprilat and low-power red light irradiation. Membrane fluidity was determined using fluorescent labels spectroscopy; membrane potential was evaluated by means of potential-sensitive fluorescent dyes; and acetylcholinesterase activity was estimated using the Ellman method. Both perindoprilat and laser irradiation, when used separately, increase microviscosity in the polar region and hyperpolarize the membranes in comparison with control erythrocytes. The combined action of these agents does not cause any further change in these parameters. Perindoprilat has an additional inhibitory effect on the activity of acetylcholinesterase, whereas laser irradiation causes an increase in the activity of the enzyme. Their combined action restores the initial activity of the enzyme independently of the sequence of treatment with both agents.
ISSN:0036-5513
1502-7686
DOI:10.1080/003655100750019305