Keratinocyte growth factor protects against Pseudomonas aeruginosa-induced lung injury

• Published in: American journal of physiology. Lung cellular and molecular physiology 2000-12, Vol.279 (6), p.1199-L1209
• Main Authors: Viget, Nathalie B, Guery, Benoit P. H, Ader, Florence, Neviere, Remi, Alfandari, Serge, Creuzy, Colette, Roussel-Delvallez, Micheline, Foucher, Claude, Mason, Carol M, Beaucaire, Gilles, Pittet, Jean-Francois
• Format: Article
• Language: English
• Subjects: Albumins - pharmacokinetics; Animals; Body Fluids - metabolism; Bronchoalveolar Lavage Fluid - cytology; Bronchoalveolar Lavage Fluid - immunology; Bronchoalveolar Lavage Fluid - microbiology; Disease Models, Animal; Fibroblast Growth Factor 10; Fibroblast Growth Factor 7; Fibroblast Growth Factors; Growth Substances - pharmacology; Iodine Radioisotopes; Macrophages, Alveolar - immunology; Macrophages, Alveolar - microbiology; Pneumonia, Bacterial - drug therapy; Pneumonia, Bacterial - mortality; Pneumonia, Bacterial - pathology; Pseudomonas aeruginosa; Pseudomonas Infections - drug therapy; Pseudomonas Infections - mortality; Pseudomonas Infections - pathology; Pulmonary Alveoli - metabolism; Pulmonary Alveoli - microbiology; Pulmonary Alveoli - pathology; Rats; Rats, Sprague-Dawley; Specific Pathogen-Free Organisms; Survival Rate
• ISSN: 1040-0605; 1522-1504
• DOI: 10.1152/ajplung.2000.279.6.L1199

1  Laboratoire de Recherche en Pathologie Infectieuse, EA 2689, 59045 Lille; Laboratoire de 4  Biophysique and 3  Bactériologie, Hôpital Calmette, Centre Hospitalier Regional Universitaire de Lille, 59037 Lille; 2  Service d'Anatomie et de Cytologie Pathologiques, Faculté Libre de Médecine, Université Catholique de Lille, 59000 Lille, France; 6  Department of Anesthesia and Perioperative Care, University of California, San Francisco, California 94143-0130; and 5  Department of Medicine, Louisiana State University Medical Center, New Orleans, Louisiana 70112 We have previously reported that keratinocyte growth factor (KGF) attenuates -naphthylthiourea-induced lung injury by upregulating alveolar fluid transport. The objective of this study was to determine the effect of KGF pretreatment in Pseudomonas aeruginosa pneumonia. A 5% bovine albumin solution with 1 µCi of 125 I-labeled human albumin was instilled into the air spaces 4 or 24 h after intratracheal instillation of P. aeruginosa , and the concentration of unlabeled and labeled proteins in the distal air spaces over 1 h was used as an index of net alveolar fluid clearance. Alveolocapillary barrier permeability was evaluated with an intravascular injection of 1 µCi of 131 I-albumin. In early pneumonia, KGF increased lung liquid clearance (LLC) compared with that in nonpretreated animals. In late pneumonia, LLC was significantly reduced in the absence of KGF but increased above the control value with KGF. KGF pretreatment increased the number of polymorphonuclear cells recovered in the bronchoalveolar lavage fluid and decreased bacterial pulmonary translocation. In conclusion, KGF restores normal alveolar epithelial fluid transport during the acute phase of P. aeruginosa pneumonia and LLC in early and late pneumonia. Host response is also improved as shown by the increase in the alveolar cellular response and the decrease in pulmonary translocation of bacteria. pneumonia; lung liquid clearance