Lack of Cross-Reactivity of Anti-DNA Antibodies to Ribonucleoproteins in a New Commercial Blot System for Specific ANAs

Anti-DNA antibodies often exhibit cross-reactivity. It has been observed that anti-DNA antibodies cross-react with A and D snRNP proteins in an in-house developed Western blot assay but do not cross-react with native U[RNP in ELISA or immunoprecipitation experiments. We have analyzed the cross-react...

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Bibliographic Details
Published in:Autoimmunity (Chur, Switzerland) Switzerland), 2000-01, Vol.32 (2), p.129-132
Main Authors: González, Concepción, Pascual, María J., González-Buitrago, Jose M.
Format: Article
Language:English
Subjects:
anti-DNA antibodies
anti-Sm
anti-U| snRNP
Antibodies, Antinuclear - immunology
Antibody Specificity
Biological and medical sciences
Blotting
Blotting, Western - methods
Cross Reactions
ELISA
Enzyme-Linked Immunosorbent Assay
Humans
Immunological methods for diagnosis and exploration
Immunopathology
Lupus Erythematosus, Systemic - immunology
Medical sciences
Ribonucleoprotein, U1 Small Nuclear - immunology
Serological tests
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Summary:Anti-DNA antibodies often exhibit cross-reactivity. It has been observed that anti-DNA antibodies cross-react with A and D snRNP proteins in an in-house developed Western blot assay but do not cross-react with native U[RNP in ELISA or immunoprecipitation experiments. We have analyzed the cross-reactivity of anti-DNA antibodies to snRNP A and D in a recently developed commercial blot assay (InnoLIA, Innogenetics). In our experience anti-DNA antibodies do not cross-react with proteins A and D in this blot system. Hence this new blot system avoids the cross-reactivity problems in the assessment of antinuclear antibody specificity for both Sm and U1snRNP
ISSN:0891-6934
1607-842X
DOI:10.3109/08916930008994082