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Cell cycle-related changes in transient K+ current density in the GH3 pituitary cell line
Laboratoire de Neurophysiologie, Centre National de la Recherche Scientifique UMR 5543, Université de Bordeaux 2, 33076 Bordeaux Cedex, France Our aim was to determine whether the expression of K + currents is related to the cell cycle in the excitable GH3 pituitary cell line. K + currents were stud...
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| Published in: | American Journal of Physiology: Cell Physiology 2000-12, Vol.279 (6), p.C1819-C1828 |
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| cited_by | cdi_FETCH-LOGICAL-c401t-967a234eacf140eea55c1f3e91307db54608aa5c0c926896d5cc70bded13f1793 |
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| cites | cdi_FETCH-LOGICAL-c401t-967a234eacf140eea55c1f3e91307db54608aa5c0c926896d5cc70bded13f1793 |
| container_end_page | C1828 |
| container_issue | 6 |
| container_start_page | C1819 |
| container_title | American Journal of Physiology: Cell Physiology |
| container_volume | 279 |
| creator | Czarnecki, A Vaur, S Dufy-Barbe, L Dufy, B Bresson-Bepoldin, L |
| description | Laboratoire de Neurophysiologie, Centre National de la Recherche
Scientifique UMR 5543, Université de Bordeaux 2, 33076 Bordeaux Cedex, France
Our aim was to determine
whether the expression of K + currents is related to the
cell cycle in the excitable GH3 pituitary cell line. K +
currents were studied by electrophysiology, and bromodeoxyuridine (BrdU) labeling was used to compare their expression in cells thereafter identified as being in the S or non-S phase of the cell
cycle. We show that the peak density of the transient outward K + current ( I to ) was 33% lower in
cells in S phase (BrdU+) than in cells in other phases of the cell
cycle (BrdU ). The voltage-dependence of I to
was not modified. However, of the two kinetic components of
I to inactivation, the characteristics of the
fast component differed significantly between BrdU+ and BrdU cells.
Recovery from inactivation of I to showed
biexponential and monoexponential function in BrdU and BrdU+ cells,
respectively. This suggests that the molecular basis of this current
varies during the cell cycle. We further demonstrated that
4-aminopyridine, which blocks I to , inhibited GH3
cell proliferation without altering the membrane potential. These data
suggest that I to may play a role in GH3 cell
proliferation processes.
potassium current; excitable cells; cell growth |
| doi_str_mv | 10.1152/ajpcell.2000.279.6.c1819 |
| format | article |
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Scientifique UMR 5543, Université de Bordeaux 2, 33076 Bordeaux Cedex, France
Our aim was to determine
whether the expression of K + currents is related to the
cell cycle in the excitable GH3 pituitary cell line. K +
currents were studied by electrophysiology, and bromodeoxyuridine (BrdU) labeling was used to compare their expression in cells thereafter identified as being in the S or non-S phase of the cell
cycle. We show that the peak density of the transient outward K + current ( I to ) was 33% lower in
cells in S phase (BrdU+) than in cells in other phases of the cell
cycle (BrdU ). The voltage-dependence of I to
was not modified. However, of the two kinetic components of
I to inactivation, the characteristics of the
fast component differed significantly between BrdU+ and BrdU cells.
Recovery from inactivation of I to showed
biexponential and monoexponential function in BrdU and BrdU+ cells,
respectively. This suggests that the molecular basis of this current
varies during the cell cycle. We further demonstrated that
4-aminopyridine, which blocks I to , inhibited GH3
cell proliferation without altering the membrane potential. These data
suggest that I to may play a role in GH3 cell
proliferation processes.
potassium current; excitable cells; cell growth</description><identifier>ISSN: 0363-6143</identifier><identifier>EISSN: 1522-1563</identifier><identifier>DOI: 10.1152/ajpcell.2000.279.6.c1819</identifier><identifier>PMID: 11078697</identifier><language>eng</language><publisher>United States</publisher><subject>4-Aminopyridine - pharmacology ; Antimetabolites ; Bromodeoxyuridine ; Cell Division - physiology ; Cell Line ; Charybdotoxin - pharmacology ; Kinetics ; Membrane Potentials - drug effects ; Membrane Potentials - physiology ; Patch-Clamp Techniques ; Pituitary Gland - cytology ; Potassium - metabolism ; Potassium Channels - metabolism ; S Phase - physiology ; Tetraethylammonium - pharmacology ; Thymidine - pharmacokinetics ; Tritium</subject><ispartof>American Journal of Physiology: Cell Physiology, 2000-12, Vol.279 (6), p.C1819-C1828</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-967a234eacf140eea55c1f3e91307db54608aa5c0c926896d5cc70bded13f1793</citedby><cites>FETCH-LOGICAL-c401t-967a234eacf140eea55c1f3e91307db54608aa5c0c926896d5cc70bded13f1793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11078697$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Czarnecki, A</creatorcontrib><creatorcontrib>Vaur, S</creatorcontrib><creatorcontrib>Dufy-Barbe, L</creatorcontrib><creatorcontrib>Dufy, B</creatorcontrib><creatorcontrib>Bresson-Bepoldin, L</creatorcontrib><title>Cell cycle-related changes in transient K+ current density in the GH3 pituitary cell line</title><title>American Journal of Physiology: Cell Physiology</title><addtitle>Am J Physiol Cell Physiol</addtitle><description>Laboratoire de Neurophysiologie, Centre National de la Recherche
Scientifique UMR 5543, Université de Bordeaux 2, 33076 Bordeaux Cedex, France
Our aim was to determine
whether the expression of K + currents is related to the
cell cycle in the excitable GH3 pituitary cell line. K +
currents were studied by electrophysiology, and bromodeoxyuridine (BrdU) labeling was used to compare their expression in cells thereafter identified as being in the S or non-S phase of the cell
cycle. We show that the peak density of the transient outward K + current ( I to ) was 33% lower in
cells in S phase (BrdU+) than in cells in other phases of the cell
cycle (BrdU ). The voltage-dependence of I to
was not modified. However, of the two kinetic components of
I to inactivation, the characteristics of the
fast component differed significantly between BrdU+ and BrdU cells.
Recovery from inactivation of I to showed
biexponential and monoexponential function in BrdU and BrdU+ cells,
respectively. This suggests that the molecular basis of this current
varies during the cell cycle. We further demonstrated that
4-aminopyridine, which blocks I to , inhibited GH3
cell proliferation without altering the membrane potential. These data
suggest that I to may play a role in GH3 cell
proliferation processes.
potassium current; excitable cells; cell growth</description><subject>4-Aminopyridine - pharmacology</subject><subject>Antimetabolites</subject><subject>Bromodeoxyuridine</subject><subject>Cell Division - physiology</subject><subject>Cell Line</subject><subject>Charybdotoxin - pharmacology</subject><subject>Kinetics</subject><subject>Membrane Potentials - drug effects</subject><subject>Membrane Potentials - physiology</subject><subject>Patch-Clamp Techniques</subject><subject>Pituitary Gland - cytology</subject><subject>Potassium - metabolism</subject><subject>Potassium Channels - metabolism</subject><subject>S Phase - physiology</subject><subject>Tetraethylammonium - pharmacology</subject><subject>Thymidine - pharmacokinetics</subject><subject>Tritium</subject><issn>0363-6143</issn><issn>1522-1563</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNp1kEFLwzAYhoMobk7_guQurUnTpo03GW4TB17mwVPI0q9rRtaVpEX7723dZHrwlI987_Py8SCEKQkpTaJ7ta01WBtGhJAwSkXIQ00zKs7QuF9HAU04O0djwjgLOI3ZCF15v-3DccTFJRpRStKMi3SM3qd9D9adthA4sKqBHOtSVRvw2FS4caryBqoGv9xh3To3jDn0f033vS8BzxcM16ZpTaNch4e7sDUVXKOLQlkPN8d3gt5mT6vpIli-zp-nj8tAx4Q2geCpilgMShc0JgAqSTQtGAjKSJqvk5iTTKlEEy0ingmeJ1qnZJ1DTllBU8EmKDv0arf33kEha2d2_SmSEjnYkkdbcrAle1uSy-lgq0dvD2jdrneQn8Cjnj4QHgKl2ZQfxoGsy86bvd1vulPtn8aH_4FZa-0KPpsf8hco67xgX6DZjjU</recordid><startdate>20001201</startdate><enddate>20001201</enddate><creator>Czarnecki, A</creator><creator>Vaur, S</creator><creator>Dufy-Barbe, L</creator><creator>Dufy, B</creator><creator>Bresson-Bepoldin, L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20001201</creationdate><title>Cell cycle-related changes in transient K+ current density in the GH3 pituitary cell line</title><author>Czarnecki, A ; Vaur, S ; Dufy-Barbe, L ; Dufy, B ; Bresson-Bepoldin, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-967a234eacf140eea55c1f3e91307db54608aa5c0c926896d5cc70bded13f1793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>4-Aminopyridine - pharmacology</topic><topic>Antimetabolites</topic><topic>Bromodeoxyuridine</topic><topic>Cell Division - physiology</topic><topic>Cell Line</topic><topic>Charybdotoxin - pharmacology</topic><topic>Kinetics</topic><topic>Membrane Potentials - drug effects</topic><topic>Membrane Potentials - physiology</topic><topic>Patch-Clamp Techniques</topic><topic>Pituitary Gland - cytology</topic><topic>Potassium - metabolism</topic><topic>Potassium Channels - metabolism</topic><topic>S Phase - physiology</topic><topic>Tetraethylammonium - pharmacology</topic><topic>Thymidine - pharmacokinetics</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Czarnecki, A</creatorcontrib><creatorcontrib>Vaur, S</creatorcontrib><creatorcontrib>Dufy-Barbe, L</creatorcontrib><creatorcontrib>Dufy, B</creatorcontrib><creatorcontrib>Bresson-Bepoldin, L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>American Journal of Physiology: Cell Physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Czarnecki, A</au><au>Vaur, S</au><au>Dufy-Barbe, L</au><au>Dufy, B</au><au>Bresson-Bepoldin, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell cycle-related changes in transient K+ current density in the GH3 pituitary cell line</atitle><jtitle>American Journal of Physiology: Cell Physiology</jtitle><addtitle>Am J Physiol Cell Physiol</addtitle><date>2000-12-01</date><risdate>2000</risdate><volume>279</volume><issue>6</issue><spage>C1819</spage><epage>C1828</epage><pages>C1819-C1828</pages><issn>0363-6143</issn><eissn>1522-1563</eissn><abstract>Laboratoire de Neurophysiologie, Centre National de la Recherche
Scientifique UMR 5543, Université de Bordeaux 2, 33076 Bordeaux Cedex, France
Our aim was to determine
whether the expression of K + currents is related to the
cell cycle in the excitable GH3 pituitary cell line. K +
currents were studied by electrophysiology, and bromodeoxyuridine (BrdU) labeling was used to compare their expression in cells thereafter identified as being in the S or non-S phase of the cell
cycle. We show that the peak density of the transient outward K + current ( I to ) was 33% lower in
cells in S phase (BrdU+) than in cells in other phases of the cell
cycle (BrdU ). The voltage-dependence of I to
was not modified. However, of the two kinetic components of
I to inactivation, the characteristics of the
fast component differed significantly between BrdU+ and BrdU cells.
Recovery from inactivation of I to showed
biexponential and monoexponential function in BrdU and BrdU+ cells,
respectively. This suggests that the molecular basis of this current
varies during the cell cycle. We further demonstrated that
4-aminopyridine, which blocks I to , inhibited GH3
cell proliferation without altering the membrane potential. These data
suggest that I to may play a role in GH3 cell
proliferation processes.
potassium current; excitable cells; cell growth</abstract><cop>United States</cop><pmid>11078697</pmid><doi>10.1152/ajpcell.2000.279.6.c1819</doi></addata></record> |
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| identifier | ISSN: 0363-6143 |
| ispartof | American Journal of Physiology: Cell Physiology, 2000-12, Vol.279 (6), p.C1819-C1828 |
| issn | 0363-6143 1522-1563 |
| language | eng |
| recordid | cdi_pubmed_primary_11078697 |
| source | American Physiological Society Free |
| subjects | 4-Aminopyridine - pharmacology Antimetabolites Bromodeoxyuridine Cell Division - physiology Cell Line Charybdotoxin - pharmacology Kinetics Membrane Potentials - drug effects Membrane Potentials - physiology Patch-Clamp Techniques Pituitary Gland - cytology Potassium - metabolism Potassium Channels - metabolism S Phase - physiology Tetraethylammonium - pharmacology Thymidine - pharmacokinetics Tritium |
| title | Cell cycle-related changes in transient K+ current density in the GH3 pituitary cell line |
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