Escherichia coli strain with a deletion of the chromosomal ampC gene marked with TcR, suitable for production of penicillin G acylase

Escherichia coli strain which contains a marker of tetracycline resistance gene (TcR) placed by P1 transduction beside the chromosomal deletion of ampC gene (delta ampC) coding for beta-lactamase was constructed. Such introduction of TcR marker permits a fast and simple selection for the transfer of...

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Bibliographic Details
Published in:Folia microbiologica 1999, Vol.44 (6), p.625-628
Main Authors: VIZVARYOVA, M, STUCHLIK, S, GRONES, J, MACOR, M, TURNA, J
Format: Article
Language:English
Subjects:
Bacterial Proteins
Bacteriology
Bacteriophage P1 - genetics
beta-Lactamases - genetics
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Escherichia coli - drug effects
Escherichia coli - enzymology
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Gene Deletion
Genes, Bacterial
Genetic Markers
Genetics
Microbiology
Mission oriented research
Penicillin Amidase - biosynthesis
Penicillin Amidase - genetics
Plasmids - genetics
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Tetracycline Resistance - genetics
Transduction, Genetic
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Summary:Escherichia coli strain which contains a marker of tetracycline resistance gene (TcR) placed by P1 transduction beside the chromosomal deletion of ampC gene (delta ampC) coding for beta-lactamase was constructed. Such introduction of TcR marker permits a fast and simple selection for the transfer of delta ampC by P1 transduction into industrial E. coli strains. This approach was used for constructing an E. coli strain suitable for penicillin acylase production.
ISSN:0015-5632
1874-9356