Radiation induced peroxidation of polyunsaturated fatty acids: Recent results on formation of hydroperoxides

• Published in: Canadian journal of physiology and pharmacology 2001-02, Vol.79 (2), p.176-179
• Main Authors: Hauville, C, Rémita, S, Thérond, P, Jore, D, Gardès-Albert, M
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biological effects of radiation; Chromatography, High Pressure Liquid; Fatty acids; Fatty Acids, Unsaturated - chemistry; Fatty Acids, Unsaturated - radiation effects; Fundamental and applied biological sciences. Psychology; Gamma Rays; Ionizing radiations; Linoleic Acid - chemistry; Linoleic Acid - radiation effects; Lipid Peroxidation - radiation effects; Lipid Peroxides - analysis; Lipid Peroxides - chemistry; Lipid Peroxides - radiation effects; Luminescent Measurements; Oxidation; Radiation; Spectrophotometry, Ultraviolet; Tissues, organs and organisms biophysics
• ISSN: 0008-4212; 1205-7541
• DOI: 10.1139/y00-086

Aqueous solutions of linoleic acid were irradiated in air with γ-rays of 137 Cs. High pressure liquid chromatography (HPLC) was been used to separate and measure the production of hydroperoxides. The results obtained after reverse phase chromatography, associated with a microperoxydase for hydroperoxide detection, indicate the presence of two different hydroperoxides. One type of hydroperoxide was the major product obtained when the initial linoleic concentrations were below the critical micellar concentration (2 mM), and the second type was produced when the concentrations were above 2 mM. A further separation carried out on the second hydroperoxide by direct phase HPLC showed that it contains three compounds, mainly HPODE 9 and 13.Key words: linoleic acid, peroxidation, hydroperoxide, direct phase HPLC, reverse phase HPLC.