Micellar electrokinetic capillary chromatography quantification of cytosine arabinoside and its metabolite, uracil arabinoside, in human serum

Cytosine arabinoside (Ara-C) is widely used to induce remission in adult granulocytic leukemia. High doses can be infused in refractory leukemia or in relapse. After injection, Ara-C is quickly metabolized to uracil arabinoside (Ara-U), the main inactive metabolite. We here described a micellar elec...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 2001-04, Vol.754 (1), p.185-192
Main Authors: Houzé, Pascal, Deschamps, Florence, Dombret, Hervé, Bousquet, Bernard, Gourmel, Bernard
Format: Article
Language:English
Subjects:
Analysis
Antimetabolites, Antineoplastic - blood
Antimetabolites, Antineoplastic - therapeutic use
Arabinofuranosyluracil - blood
Biological and medical sciences
Chromatography, Micellar Electrokinetic Capillary - methods
Cytarabine - blood
Cytarabine - isolation & purification
Cytarabine - therapeutic use
Cytosine arabinoside
General pharmacology
Humans
Leukemia, Myeloid - drug therapy
Medical sciences
Osmolar Concentration
Pharmacology. Drug treatments
Reproducibility of Results
Sensitivity and Specificity
Temperature
Uracil arabinoside
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Summary:Cytosine arabinoside (Ara-C) is widely used to induce remission in adult granulocytic leukemia. High doses can be infused in refractory leukemia or in relapse. After injection, Ara-C is quickly metabolized to uracil arabinoside (Ara-U), the main inactive metabolite. We here described a micellar electrokinetic capillary chromatography (MECC) method to simultaneously determine Ara-C/Ara-U in human serum using 6- O-methylguanine as an internal standard. The assay was linear from 6.25 to 200 μg/ml with a quantification limit between 3 and 6 μg/ml. The analytical precision was satisfactory between 2 and 4.3% (within-run) and 3.7 and 7.3% (between-runs). This assay was applied to the analysis of serum from acute granulocytic leukemia patient treated by high doses cytarabine (3 g/m 2 body surface).
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(00)00605-8