A novel dual-specificity protein kinase targeted to the chloroplast in tobacco

The NtDSK1 cDNA encoding a novel chloroplast-targeted protein kinase was identified in Nicotiana tabacum. It contains the kinase domain at the C-terminus and a putative regulatory domain at the N-terminus. The recombinant NtDSK1 underwent autophosphorylation of serine, threonine, and tyrosine residu...

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Bibliographic Details
Published in:FEBS letters 2001-05, Vol.497 (2), p.124-130
Main Authors: Cho, Hye Sun, Yoon, Gyeong Mee, Lee, Sang Sook, Kim, Young A., Hwang, Inhwan, Choi, Doil, Pai, Hyun-Sook
Format: Article
Language:English
Subjects:
Chloroplast-targeted
Chloroplasts - enzymology
Chloroplasts - radiation effects
DNA, Complementary - genetics
Dual-specificity protein kinase
Gene Expression Regulation, Plant - drug effects
Gene Expression Regulation, Plant - radiation effects
Genomic Library
Gibberellins - pharmacology
Light
Light- and gibberellin-stimulated expression
Molecular Sequence Data
Nicotiana - enzymology
NtDSK1
Phosphorylation
Plant Structures - enzymology
Plants, Toxic
Protein Kinases - genetics
Protein Kinases - metabolism
Protein Serine-Threonine Kinases - genetics
Protein Serine-Threonine Kinases - metabolism
Protein-Tyrosine Kinases - genetics
Protein-Tyrosine Kinases - metabolism
RNA, Messenger - metabolism
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Substrate Specificity - physiology
Tobacco
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Summary:The NtDSK1 cDNA encoding a novel chloroplast-targeted protein kinase was identified in Nicotiana tabacum. It contains the kinase domain at the C-terminus and a putative regulatory domain at the N-terminus. The recombinant NtDSK1 underwent autophosphorylation of serine, threonine, and tyrosine residues, indicating that NtDSK1 encodes a functional dual-specificity protein kinase. The NtDSK1–green fluorescent protein fusion protein was targeted to chloroplasts. Furthermore, the NtDSK1 protein was immunodetected in chloroplast fractions isolated from tobacco seedlings. The NtDSK1 mRNA expression was developmentally regulated in different tissues, including anthers and germinating seeds, and strongly stimulated by gibberellin. The mRNA was rapidly light responsive during seedling growth. NtDSK1 may play a role in a light-regulated signaling process in tobacco.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(01)02439-5