Functional aspects of creatine kinase isoenzymes in endothelial cells

1  Institut für Herz- und Kreislaufphysiologie, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany; and 2  Institute for Cell Biology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland To characterize the isoenzyme distribution of creatine kinase (CK) in endothelial cells (ECs) and its func...

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Published in:American Journal of Physiology: Cell Physiology 2001-07, Vol.281 (1), p.C320-C328
Main Authors: Decking, Ulrich K. M, Alves, Christiane, Wallimann, Theo, Wyss, Markus, Schrader, Jurgen
Format: Article
Language:English
Subjects:
Animals
Aorta
Brain
Capillaries
Cells, Cultured
Creatine - pharmacology
Creatine Kinase - metabolism
Electrophoresis
Endothelium, Vascular - cytology
Endothelium, Vascular - drug effects
Endothelium, Vascular - enzymology
Isoenzymes - metabolism
Magnetic Resonance Spectroscopy
Male
Mitochondria - enzymology
Myocardium
Organophosphates - metabolism
Rats
Rats, Wistar
Swine
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Summary:1  Institut für Herz- und Kreislaufphysiologie, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany; and 2  Institute for Cell Biology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland To characterize the isoenzyme distribution of creatine kinase (CK) in endothelial cells (ECs) and its functional role during substrate depletion, ECs from aorta (AECs) and microvasculature (MVECs) of pig and rat were studied. In addition, high- energy phosphates were continuously monitored by 31 P NMR spectroscopy in pig AECs attached to microcarrier beads. CK activity per milligram of protein in rat AECs and MVECs (0.08   ± 0.01 and 0.15 ± 0.08 U/mg, respectively) was
ISSN:0363-6143
1522-1563
DOI:10.1152/ajpcell.2001.281.1.c320