dl-Buthionine- S, R-sulfoximine affects intestinal alkaline phosphatase activity

The susceptibility of intestinal alkaline phosphatase to dl-buthionine- S, R-sulfoximine was investigated in chicks fed a commercial diet. The results show that dl-buthionine- S, R-sulfoximine produced inhibition of intestinal alkaline phosphatase activity. This effect showed dose- and time-dependen...

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Bibliographic Details
Published in:Comparative biochemistry and physiology. Toxicology & pharmacology 2001-06, Vol.129 (2), p.85-91
Main Authors: Marchionatti, Ana, Alisio, Arturo, Dı&#x0301, az de Barboza, Gabriela, Baudino, Vanessa, Tolosa de Talamoni, Nori
Format: Article
Language:English
Subjects:
Alkaline phosphatase
Alkaline Phosphatase - metabolism
Animals
Antimetabolites - pharmacology
Buthionine Sulfoximine - administration & dosage
Buthionine Sulfoximine - pharmacology
Calcium - pharmacokinetics
Chickens - metabolism
Chicks
dl-Buthionine- S, R-sulfoximine
Dose-Response Relationship, Drug
Duodenum - enzymology
Electron Spin Resonance Spectroscopy
Free hydroxyl radicals
Free Radicals
Glutathione
Glutathione - physiology
Glutathione monoester
In Vitro Techniques
Injections, Intraperitoneal
Intestinal Absorption - physiology
Intestinal Mucosa - drug effects
Intestinal Mucosa - enzymology
intestine
Kinetics
Oxidative Stress
Spectrophotometry, Atomic
Time Factors
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Summary:The susceptibility of intestinal alkaline phosphatase to dl-buthionine- S, R-sulfoximine was investigated in chicks fed a commercial diet. The results show that dl-buthionine- S, R-sulfoximine produced inhibition of intestinal alkaline phosphatase activity. This effect showed dose- and time-dependency and it was caused by either in vivo dl-buthionine- S, R- sulfoximine administration or in vitro dl-buthionine- S, R-sulfoximine incubation with villus tip enterocytes. dl-Buthionine- S, R-sulfoximine did not act directly on intestinal alkaline phosphatase but it provoked glutathione depletion which led to changes in the redox state of the enterocyte as shown by the production of free hydroxyl radicals and an incremental increase in the carbonyl content of proteins. The reversibility of the buthionine sulfoximine effect on intestinal alkaline phosphatase was proved by addition of glutathione monoester to the duodenal loop.
ISSN:1532-0456
1878-1659
DOI:10.1016/S1532-0456(01)00185-5