DNA microarray analysis of gene expression in endothelial cells in response to 24-h shear stress

1 Department of Bioengineering and the Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla 92093-0427 2 Division of Biomedical Sciences, University of California, Riverside, Riverside, California The recently developed DNA microarray technology provides a powe...

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Bibliographic Details
Published in:Physiological genomics 2001-10, Vol.7 (1), p.55-63
Main Authors: CHEN, BENJAMIN P. C, LI, YI-SHUAN, ZHAO, YIHUA, CHEN, KUANG-DEN, LI, SONG, LAO, JIANMIN, YUAN, SULI, SHYY, JOHN Y.-J, CHIEN, SHU
Format: Article
Language:English
Subjects:
Adult
Aorta
Blood Flow Velocity
Blotting, Northern
Cell Division - genetics
Cells, Cultured
Endothelium, Vascular - cytology
Endothelium, Vascular - metabolism
Endothelium, Vascular - pathology
Female
Gene Expression Profiling
Gene Expression Regulation
Hemorheology
Humans
Inflammation - genetics
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Signal Transduction - genetics
Time Factors
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Summary:1 Department of Bioengineering and the Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla 92093-0427 2 Division of Biomedical Sciences, University of California, Riverside, Riverside, California The recently developed DNA microarray technology provides a powerful and efficient tool to rapidly compare the differential expression of a large number of genes. Using the DNA microarray approach, we investigated gene expression profiles in cultured human aortic endothelial cells (HAECs) in response to 24 h of laminar shear stress at 12 dyn/cm 2 . This relatively long-term shearing of cultured HAECs led to the modulation of the expression of a number of genes. Several genes related to inflammation and EC proliferation were downregulated, suggesting that 24-h shearing may keep ECs in a relatively noninflammatory and nonproliferative state compared with static cells. Some genes were significantly upregulated by the 24-h shear stress; these includes genes involved in EC survival and angiogenesis (Tie2 and Flk-1) and vascular remodeling (matrix metalloproteinase 1). These results provide information on the profile of gene expression in shear-adapted ECs, which is the case for the native ECs in the straight part of the aorta in vivo. DNA microarray; endothelial cells; gene expression; shear stress
ISSN:1094-8341
1531-2267
DOI:10.1152/physiolgenomics.2001.7.1.55