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γ-Radiation Sensitivity and Risk of Glioma
Background: About 9% of human cancers are brain tumors, of which 90% are gliomas. γ-Radiation has been identified as a risk factor for brain tumors. In a previous pilot study, we found that lymphocytes from patients with glioma were more sensitive to γ-radiation than were lymphocytes from matched co...
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| Published in: | JNCI : Journal of the National Cancer Institute 2001-10, Vol.93 (20), p.1553-1557 |
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| container_title | JNCI : Journal of the National Cancer Institute |
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| creator | Bondy, Melissa L. Wang, Li-E. El-Zein, Randa de Andrade, Mariza Selvan, Mano S. Bruner, Janet M. Levin, Victor A. Alfred Yung, W. K. Adatto, Phyllis Wei, Qingyi |
| description | Background: About 9% of human cancers are brain tumors, of which 90% are gliomas. γ-Radiation has been identified as a risk factor for brain tumors. In a previous pilot study, we found that lymphocytes from patients with glioma were more sensitive to γ-radiation than were lymphocytes from matched control subjects. In this larger case–control study, we compared the γ-radiation sensitivity of lymphocytes from glioma patients with those from control subjects and investigated the association between mutagen sensitivity and the risk for developing glioma. Methods: We used a mutagen sensitivity assay (an indirect measure of DNA repair activity) to assess chromosomal damage. We γ-irradiated (1.5 Gy) short-term lymphocyte cultures from 219 case patients with glioma and from 238 healthy control subjects frequency matched by age and sex. After irradiation, cells were cultured for 4 hours, and then Colcemid was added for 1 hour to arrest cells in mitosis. Fifty metaphases were randomly selected for each sample and scored for chromatid breaks. All statistical tests were two-sided. Results: We observed a statistically significantly higher frequency of chromatid breaks per cell from case patients with glioma (mean = 0.55; 95% confidence interval [CI] = 0.50 to 0.59) than from control subjects (mean = 0.44; 95% CI = 0.41 to 0.48) (P |
| doi_str_mv | 10.1093/jnci/93.20.1553 |
| format | article |
| fullrecord | <record><control><sourceid>istex_pubme</sourceid><recordid>TN_cdi_pubmed_primary_11604478</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>ark_67375_HXZ_Z677Q2SB_V</sourcerecordid><originalsourceid>FETCH-LOGICAL-i245t-86e2cf68c0f6533db1b081142e04924964616b3126dea01421a93b21b7cd53f63</originalsourceid><addsrcrecordid>eNo9TstKw0AUHUSxsbp2J9nLtHPnnaUWbYWC2KpIN8NMMoFpm6Rkotjv8j_8JgNV7-ZwHpxzEboEMgKSsfG6zsM4YyPacyHYEUqAS4IpEHGMEkKowlorPkBnMa5Jfxnlp2gAIAnnSifo-vsLL2wRbBeaOl36OoYufIRun9q6SBchbtKmTKfb0FT2HJ2Udhv9xS8O0cv93fNkhueP04fJzRwHykWHtfQ0L6XOSSkFY4UDRzQAp57wfj6TXIJ0DKgsvCW9DjZjjoJTeSFYKdkQXR16d--u8oXZtaGy7d78fd0H8CEQYuc__33bboxUTAkze1uZlVTqiS5vzSv7AannUeM</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>γ-Radiation Sensitivity and Risk of Glioma</title><source>Oxford Journals Online</source><creator>Bondy, Melissa L. ; Wang, Li-E. ; El-Zein, Randa ; de Andrade, Mariza ; Selvan, Mano S. ; Bruner, Janet M. ; Levin, Victor A. ; Alfred Yung, W. K. ; Adatto, Phyllis ; Wei, Qingyi</creator><creatorcontrib>Bondy, Melissa L. ; Wang, Li-E. ; El-Zein, Randa ; de Andrade, Mariza ; Selvan, Mano S. ; Bruner, Janet M. ; Levin, Victor A. ; Alfred Yung, W. K. ; Adatto, Phyllis ; Wei, Qingyi</creatorcontrib><description>Background: About 9% of human cancers are brain tumors, of which 90% are gliomas. γ-Radiation has been identified as a risk factor for brain tumors. In a previous pilot study, we found that lymphocytes from patients with glioma were more sensitive to γ-radiation than were lymphocytes from matched control subjects. In this larger case–control study, we compared the γ-radiation sensitivity of lymphocytes from glioma patients with those from control subjects and investigated the association between mutagen sensitivity and the risk for developing glioma. Methods: We used a mutagen sensitivity assay (an indirect measure of DNA repair activity) to assess chromosomal damage. We γ-irradiated (1.5 Gy) short-term lymphocyte cultures from 219 case patients with glioma and from 238 healthy control subjects frequency matched by age and sex. After irradiation, cells were cultured for 4 hours, and then Colcemid was added for 1 hour to arrest cells in mitosis. Fifty metaphases were randomly selected for each sample and scored for chromatid breaks. All statistical tests were two-sided. Results: We observed a statistically significantly higher frequency of chromatid breaks per cell from case patients with glioma (mean = 0.55; 95% confidence interval [CI] = 0.50 to 0.59) than from control subjects (mean = 0.44; 95% CI = 0.41 to 0.48) (P<.001). Using 0.40 (the median number of chromatid breaks per cell in control subjects) as the cut point for defining mutagen sensitivity and adjusting for age, sex, and smoking status, we found that mutagen sensitivity was statistically significantly associated with an increased risk for glioma (odds ratio = 2.09; 95% CI = 1.43 to 3.06). When the data were divided into tertiles, the relative risk for glioma increased from the lowest tertile to the highest tertile (trend test, P<.001). Conclusion: γ-Radiation-induced mutagen sensitivity of lymphocytes may be associated with an increased risk for glioma, a result that supports our earlier preliminary findings.</description><identifier>ISSN: 0027-8874</identifier><identifier>EISSN: 1460-2105</identifier><identifier>DOI: 10.1093/jnci/93.20.1553</identifier><identifier>PMID: 11604478</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Adult ; Animals ; Brain Neoplasms - epidemiology ; Brain Neoplasms - etiology ; Brain Neoplasms - genetics ; Case-Control Studies ; Chromatids - radiation effects ; Chromatids - ultrastructure ; Chromosome Breakage ; Demecolcine - pharmacology ; DNA - radiation effects ; DNA Damage ; DNA Repair - genetics ; DNA Repair - radiation effects ; DNA, Single-Stranded - radiation effects ; Female ; Gamma Rays - adverse effects ; Genetic Predisposition to Disease ; Glioma - epidemiology ; Glioma - etiology ; Glioma - genetics ; Humans ; Lymphocytes - pathology ; Lymphocytes - radiation effects ; Male ; Middle Aged ; Neoplasms, Radiation-Induced - epidemiology ; Neoplasms, Radiation-Induced - etiology ; Neoplasms, Radiation-Induced - genetics ; Odds Ratio ; Radiation Tolerance - genetics ; Risk ; Smoking - epidemiology</subject><ispartof>JNCI : Journal of the National Cancer Institute, 2001-10, Vol.93 (20), p.1553-1557</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11604478$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bondy, Melissa L.</creatorcontrib><creatorcontrib>Wang, Li-E.</creatorcontrib><creatorcontrib>El-Zein, Randa</creatorcontrib><creatorcontrib>de Andrade, Mariza</creatorcontrib><creatorcontrib>Selvan, Mano S.</creatorcontrib><creatorcontrib>Bruner, Janet M.</creatorcontrib><creatorcontrib>Levin, Victor A.</creatorcontrib><creatorcontrib>Alfred Yung, W. K.</creatorcontrib><creatorcontrib>Adatto, Phyllis</creatorcontrib><creatorcontrib>Wei, Qingyi</creatorcontrib><title>γ-Radiation Sensitivity and Risk of Glioma</title><title>JNCI : Journal of the National Cancer Institute</title><addtitle>JNCI J Natl Cancer Inst</addtitle><description>Background: About 9% of human cancers are brain tumors, of which 90% are gliomas. γ-Radiation has been identified as a risk factor for brain tumors. In a previous pilot study, we found that lymphocytes from patients with glioma were more sensitive to γ-radiation than were lymphocytes from matched control subjects. In this larger case–control study, we compared the γ-radiation sensitivity of lymphocytes from glioma patients with those from control subjects and investigated the association between mutagen sensitivity and the risk for developing glioma. Methods: We used a mutagen sensitivity assay (an indirect measure of DNA repair activity) to assess chromosomal damage. We γ-irradiated (1.5 Gy) short-term lymphocyte cultures from 219 case patients with glioma and from 238 healthy control subjects frequency matched by age and sex. After irradiation, cells were cultured for 4 hours, and then Colcemid was added for 1 hour to arrest cells in mitosis. Fifty metaphases were randomly selected for each sample and scored for chromatid breaks. All statistical tests were two-sided. Results: We observed a statistically significantly higher frequency of chromatid breaks per cell from case patients with glioma (mean = 0.55; 95% confidence interval [CI] = 0.50 to 0.59) than from control subjects (mean = 0.44; 95% CI = 0.41 to 0.48) (P<.001). Using 0.40 (the median number of chromatid breaks per cell in control subjects) as the cut point for defining mutagen sensitivity and adjusting for age, sex, and smoking status, we found that mutagen sensitivity was statistically significantly associated with an increased risk for glioma (odds ratio = 2.09; 95% CI = 1.43 to 3.06). When the data were divided into tertiles, the relative risk for glioma increased from the lowest tertile to the highest tertile (trend test, P<.001). Conclusion: γ-Radiation-induced mutagen sensitivity of lymphocytes may be associated with an increased risk for glioma, a result that supports our earlier preliminary findings.</description><subject>Adult</subject><subject>Animals</subject><subject>Brain Neoplasms - epidemiology</subject><subject>Brain Neoplasms - etiology</subject><subject>Brain Neoplasms - genetics</subject><subject>Case-Control Studies</subject><subject>Chromatids - radiation effects</subject><subject>Chromatids - ultrastructure</subject><subject>Chromosome Breakage</subject><subject>Demecolcine - pharmacology</subject><subject>DNA - radiation effects</subject><subject>DNA Damage</subject><subject>DNA Repair - genetics</subject><subject>DNA Repair - radiation effects</subject><subject>DNA, Single-Stranded - radiation effects</subject><subject>Female</subject><subject>Gamma Rays - adverse effects</subject><subject>Genetic Predisposition to Disease</subject><subject>Glioma - epidemiology</subject><subject>Glioma - etiology</subject><subject>Glioma - genetics</subject><subject>Humans</subject><subject>Lymphocytes - pathology</subject><subject>Lymphocytes - radiation effects</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neoplasms, Radiation-Induced - epidemiology</subject><subject>Neoplasms, Radiation-Induced - etiology</subject><subject>Neoplasms, Radiation-Induced - genetics</subject><subject>Odds Ratio</subject><subject>Radiation Tolerance - genetics</subject><subject>Risk</subject><subject>Smoking - epidemiology</subject><issn>0027-8874</issn><issn>1460-2105</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNo9TstKw0AUHUSxsbp2J9nLtHPnnaUWbYWC2KpIN8NMMoFpm6Rkotjv8j_8JgNV7-ZwHpxzEboEMgKSsfG6zsM4YyPacyHYEUqAS4IpEHGMEkKowlorPkBnMa5Jfxnlp2gAIAnnSifo-vsLL2wRbBeaOl36OoYufIRun9q6SBchbtKmTKfb0FT2HJ2Udhv9xS8O0cv93fNkhueP04fJzRwHykWHtfQ0L6XOSSkFY4UDRzQAp57wfj6TXIJ0DKgsvCW9DjZjjoJTeSFYKdkQXR16d--u8oXZtaGy7d78fd0H8CEQYuc__33bboxUTAkze1uZlVTqiS5vzSv7AannUeM</recordid><startdate>20011017</startdate><enddate>20011017</enddate><creator>Bondy, Melissa L.</creator><creator>Wang, Li-E.</creator><creator>El-Zein, Randa</creator><creator>de Andrade, Mariza</creator><creator>Selvan, Mano S.</creator><creator>Bruner, Janet M.</creator><creator>Levin, Victor A.</creator><creator>Alfred Yung, W. K.</creator><creator>Adatto, Phyllis</creator><creator>Wei, Qingyi</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20011017</creationdate><title>γ-Radiation Sensitivity and Risk of Glioma</title><author>Bondy, Melissa L. ; Wang, Li-E. ; El-Zein, Randa ; de Andrade, Mariza ; Selvan, Mano S. ; Bruner, Janet M. ; Levin, Victor A. ; Alfred Yung, W. K. ; Adatto, Phyllis ; Wei, Qingyi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i245t-86e2cf68c0f6533db1b081142e04924964616b3126dea01421a93b21b7cd53f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Brain Neoplasms - epidemiology</topic><topic>Brain Neoplasms - etiology</topic><topic>Brain Neoplasms - genetics</topic><topic>Case-Control Studies</topic><topic>Chromatids - radiation effects</topic><topic>Chromatids - ultrastructure</topic><topic>Chromosome Breakage</topic><topic>Demecolcine - pharmacology</topic><topic>DNA - radiation effects</topic><topic>DNA Damage</topic><topic>DNA Repair - genetics</topic><topic>DNA Repair - radiation effects</topic><topic>DNA, Single-Stranded - radiation effects</topic><topic>Female</topic><topic>Gamma Rays - adverse effects</topic><topic>Genetic Predisposition to Disease</topic><topic>Glioma - epidemiology</topic><topic>Glioma - etiology</topic><topic>Glioma - genetics</topic><topic>Humans</topic><topic>Lymphocytes - pathology</topic><topic>Lymphocytes - radiation effects</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neoplasms, Radiation-Induced - epidemiology</topic><topic>Neoplasms, Radiation-Induced - etiology</topic><topic>Neoplasms, Radiation-Induced - genetics</topic><topic>Odds Ratio</topic><topic>Radiation Tolerance - genetics</topic><topic>Risk</topic><topic>Smoking - epidemiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bondy, Melissa L.</creatorcontrib><creatorcontrib>Wang, Li-E.</creatorcontrib><creatorcontrib>El-Zein, Randa</creatorcontrib><creatorcontrib>de Andrade, Mariza</creatorcontrib><creatorcontrib>Selvan, Mano S.</creatorcontrib><creatorcontrib>Bruner, Janet M.</creatorcontrib><creatorcontrib>Levin, Victor A.</creatorcontrib><creatorcontrib>Alfred Yung, W. K.</creatorcontrib><creatorcontrib>Adatto, Phyllis</creatorcontrib><creatorcontrib>Wei, Qingyi</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>JNCI : Journal of the National Cancer Institute</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bondy, Melissa L.</au><au>Wang, Li-E.</au><au>El-Zein, Randa</au><au>de Andrade, Mariza</au><au>Selvan, Mano S.</au><au>Bruner, Janet M.</au><au>Levin, Victor A.</au><au>Alfred Yung, W. K.</au><au>Adatto, Phyllis</au><au>Wei, Qingyi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>γ-Radiation Sensitivity and Risk of Glioma</atitle><jtitle>JNCI : Journal of the National Cancer Institute</jtitle><addtitle>JNCI J Natl Cancer Inst</addtitle><date>2001-10-17</date><risdate>2001</risdate><volume>93</volume><issue>20</issue><spage>1553</spage><epage>1557</epage><pages>1553-1557</pages><issn>0027-8874</issn><eissn>1460-2105</eissn><abstract>Background: About 9% of human cancers are brain tumors, of which 90% are gliomas. γ-Radiation has been identified as a risk factor for brain tumors. In a previous pilot study, we found that lymphocytes from patients with glioma were more sensitive to γ-radiation than were lymphocytes from matched control subjects. In this larger case–control study, we compared the γ-radiation sensitivity of lymphocytes from glioma patients with those from control subjects and investigated the association between mutagen sensitivity and the risk for developing glioma. Methods: We used a mutagen sensitivity assay (an indirect measure of DNA repair activity) to assess chromosomal damage. We γ-irradiated (1.5 Gy) short-term lymphocyte cultures from 219 case patients with glioma and from 238 healthy control subjects frequency matched by age and sex. After irradiation, cells were cultured for 4 hours, and then Colcemid was added for 1 hour to arrest cells in mitosis. Fifty metaphases were randomly selected for each sample and scored for chromatid breaks. All statistical tests were two-sided. Results: We observed a statistically significantly higher frequency of chromatid breaks per cell from case patients with glioma (mean = 0.55; 95% confidence interval [CI] = 0.50 to 0.59) than from control subjects (mean = 0.44; 95% CI = 0.41 to 0.48) (P<.001). Using 0.40 (the median number of chromatid breaks per cell in control subjects) as the cut point for defining mutagen sensitivity and adjusting for age, sex, and smoking status, we found that mutagen sensitivity was statistically significantly associated with an increased risk for glioma (odds ratio = 2.09; 95% CI = 1.43 to 3.06). When the data were divided into tertiles, the relative risk for glioma increased from the lowest tertile to the highest tertile (trend test, P<.001). Conclusion: γ-Radiation-induced mutagen sensitivity of lymphocytes may be associated with an increased risk for glioma, a result that supports our earlier preliminary findings.</abstract><cop>United States</cop><pub>Oxford University Press</pub><pmid>11604478</pmid><doi>10.1093/jnci/93.20.1553</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0027-8874 |
| ispartof | JNCI : Journal of the National Cancer Institute, 2001-10, Vol.93 (20), p.1553-1557 |
| issn | 0027-8874 1460-2105 |
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| source | Oxford Journals Online |
| subjects | Adult Animals Brain Neoplasms - epidemiology Brain Neoplasms - etiology Brain Neoplasms - genetics Case-Control Studies Chromatids - radiation effects Chromatids - ultrastructure Chromosome Breakage Demecolcine - pharmacology DNA - radiation effects DNA Damage DNA Repair - genetics DNA Repair - radiation effects DNA, Single-Stranded - radiation effects Female Gamma Rays - adverse effects Genetic Predisposition to Disease Glioma - epidemiology Glioma - etiology Glioma - genetics Humans Lymphocytes - pathology Lymphocytes - radiation effects Male Middle Aged Neoplasms, Radiation-Induced - epidemiology Neoplasms, Radiation-Induced - etiology Neoplasms, Radiation-Induced - genetics Odds Ratio Radiation Tolerance - genetics Risk Smoking - epidemiology |
| title | γ-Radiation Sensitivity and Risk of Glioma |
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