Electrical stimulation of transforming growth factor-beta 1 secretion by human dermal fibroblasts and the U937 human monocytic cell line

The in vitro effects on human dermal fibroblasts and the U937 human monocytic cell line of three phases of electrical microcurrents generated by the ACE Stimulator were investigated. The growth and viability of growing and confluent dermal fibroblasts were not directly influenced by the separate mic...

Full description

Saved in:
Bibliographic Details
Published in:Alternatives to laboratory animals 2001-11, Vol.29 (6), p.693
Main Authors: Todd, I, Clothier, R H, Huggins, M L, Patel, N, Searle, K C, Jeyarajah, S, Pradel, L, Lacey, K L
Format: Article
Language:English
Subjects:
Animal Testing Alternatives
Cell Survival
Dermis - cytology
Dermis - drug effects
Dermis - metabolism
Electric Stimulation - adverse effects
Enzyme-Linked Immunosorbent Assay
Fibroblasts - cytology
Fibroblasts - drug effects
Fibroblasts - metabolism
Humans
Interleukin-6 - metabolism
Mitochondria - metabolism
Monocytes - cytology
Monocytes - drug effects
Monocytes - metabolism
Tetradecanoylphorbol Acetate - pharmacology
Transforming Growth Factor beta - metabolism
Transforming Growth Factor beta1
U937 Cells
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The in vitro effects on human dermal fibroblasts and the U937 human monocytic cell line of three phases of electrical microcurrents generated by the ACE Stimulator were investigated. The growth and viability of growing and confluent dermal fibroblasts were not directly influenced by the separate microcurrent phases. One form of microcurrent (designated phase 1) stimulated both dermal fibroblasts and U937 cells to secrete transforming growth factor-beta 1 (TGF-beta 1), which is an important regulator of cell-mediated inflammation and tissue regeneration, but none of the three phases stimulated secretion of the pro-inflammatory cytokine interleukin-6 by U937 cells. The stimulation of TGF-beta 1 secretion in these experiments was not dramatic (a median increase over control levels of 20-30%), although it could be biologically significant.
ISSN:0261-1929
DOI:10.1177/026119290102900611