Transfer of Vitamins E and A from Yolk to Embryo during Development of the King Penguin (Aptenodytes patagonicus)

• Published in: Physiological and biochemical zoology 2001-11, Vol.74 (6), p.928-936
• Main Authors: Surai, Peter F., Speake, Brian K., Decrock, Frederic, Groscolas, René
• Format: Article
• Language: English
• Subjects: Adaptation, Physiological; Animals; Antioxidants; Birds - physiology; Brain - physiology; Carotenoids; Egg Yolk - physiology; Eggs; Embryo, Nonmammalian - physiology; Embryos; Esters; Fatty Acids; Female; Hatching; Liver; Liver - chemistry; Male; Oxidative Stress; Penguins; Tissue Distribution; Vitamin A; Vitamin A - pharmacokinetics; Vitamin E; Vitamin E - pharmacokinetics
• ISSN: 1522-2152; 1537-5293
• DOI: 10.1086/338062

Since the yolk lipids of the king penguin (Aptenodytes patagonicus) are rich in n‐3 fatty acids, which are potentially susceptible to peroxidative damage, the yolk contents and yolk‐to‐embryo transfer of antioxidants and lipid‐soluble vitamins were investigated under conditions of natural incubation in the wild. The concentration of vitamin E in the unincubated egg was 155 μg/g wet yolk, of which 88% was α‐tocopherol and the rest was γ‐tocopherol. Vitamin A (2.9 μg/g) was present in the yolk entirely as retinol; no retinyl esters were detected. Throughout the latter half of the incubation period, vitamins E and A were taken up from the yolk into the yolk sac membrane (YSM) and later accumulated in the liver, with vitamin A being transferred in advance of vitamin E. In the YSM, vitamin A was present almost entirely as retinyl ester, indicating that the free retinol of the yolk is rapidly esterified following uptake. Retinyl esters were also the predominant form in the liver. The retinyl esters of the liver and YSM displayed different fatty acid profiles. At hatching, the brain contained relatively little vitamin E (4.7 μg/g) compared to the much higher concentration in the liver (482.9 μg/g) at this stage. Ascorbic acid was not detected in the yolk but was present at a high concentration in the brain at day 27 (404.6 μg/g), decreasing to less than half this value by the time of hatching. This report is the first to delineate the yolk‐to‐embryo transfer of lipid‐soluble vitamins for a free‐living avian species. The yolk fatty acids of the king penguin provide an extreme example of potential oxidative susceptibility, forming a basis for comparative studies on embryonic antioxidant requirements among species of birds whose yolk lipids differ in their degree of unsaturation.