Roles for kinesin and myosin during cytokinesis

Cytokinesis in higher plants involves the phragmoplast, a complex cytoplasmic structure that consists of microtubules (MTs), microfilaments (MFs) and membrane elements. Both MTs and MFs are essential for cell plate formation, although it is not clear which motor proteins are involved. Some candidate...

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Bibliographic Details
Published in:Philosophical transactions of the Royal Society of London. Series B. Biological sciences 2002-06, Vol.357 (1422), p.761-766
Main Authors: Hepler, Peter K., Valster, Aline, Molchan, Tasha, Vos, Jan W.
Format: Article
Language:English
Subjects:
2,3-butanedione monoxime
actin
Actin Microfilaments
Actins
Anaphase
Animal cells
Antibodies
arabidopsis
Arabidopsis Proteins - physiology
calmodulin-binding protein
Calmodulin-Binding Proteins - physiology
Cell Division - physiology
Cell walls
cell-division
Cytokinesis
Hair cells
higher-plants
inhibition
Kinesin
Kinesin - physiology
localization
Microtubules
Mother cells
Myosin
Myosins - physiology
organization
Phragmoplast
Plant Cells
Plant Physiological Phenomena
Plants
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Summary:Cytokinesis in higher plants involves the phragmoplast, a complex cytoplasmic structure that consists of microtubules (MTs), microfilaments (MFs) and membrane elements. Both MTs and MFs are essential for cell plate formation, although it is not clear which motor proteins are involved. Some candidate processes for motor proteins include transport of Golgi vesicles to the plane of the cell plate and the spatiotemporal organization of the cytoskeletal elements in order to achieve proper deposition and alignment of the cell plate. We have focused on the kinesin-like calmodulin binding protein (KCBP) and, more broadly, on myosins. Using an antibody that constitutively activates KCBP, we find that this MT motor, which is minus-end directed, contributes to the organization of the spindle and phragmoplast MTs. It does not participate in vesicle transport; rather, because of the orientation of the phragmoplast MTs, it is supposed that plus-end kinesins fill this role. Myosins, on the other hand, based on their inhibition with 2,3-butanedione monoxime and 1-(5-iodonaphthalene-1-sulphonyl)-1H-hexahydro-1,4-diazepine (ML-7), are associated with the process of post-mitotic spindle/phragmoplast alignment and with late lateral expansion of the cell plate. They are also not the principal motors involved in vesicle transport.
ISSN:0962-8436
1471-2970
DOI:10.1098/rstb.2002.1093