Differential COX localization and PG release in Thy-1+ and Thy-1- human female reproductive tract fibroblasts

• Published in: American Journal of Physiology: Cell Physiology 2002-08, Vol.283 (2), p.C599-C608
• Main Authors: Koumas, Laura, Phipps, Richard P
• Format: Article
• Language: English
• Subjects: Adult; Blotting, Western; Cells, Cultured; Cyclooxygenase 1; Cyclooxygenase 2; Female; Fibroblasts - classification; Fibroblasts - metabolism; Genitalia, Female - cytology; Genitalia, Female - metabolism; Humans; Immunohistochemistry; Isoenzymes - genetics; Isoenzymes - metabolism; Membrane Proteins; Mitogen-Activated Protein Kinases - physiology; Myometrium - cytology; Myometrium - metabolism; p38 Mitogen-Activated Protein Kinases; Prostaglandin-Endoperoxide Synthases - genetics; Prostaglandin-Endoperoxide Synthases - metabolism; Prostaglandins - metabolism; RNA, Messenger - metabolism; Signal Transduction - physiology; Thy-1 Antigens - metabolism
• ISSN: 0363-6143; 1522-1563
• DOI: 10.1152/ajpcell.00065.2002

Departments of 1  Microbiology and Immunology, 2  Environmental Medicine, and 3  Pediatrics and Obstetrics and Gynecology, University of Rochester School of Medicine and Dentistry, Lung Biology and Disease Program, Rochester, New York 14642 A key role exists for prostaglandins (PGs) in reproductive health, including fertility and parturition. However, the cellular sources and regulation of PG production by cyclooxygenase (COX) in the human female reproductive tract remain poorly understood. We recently reported that human female reproductive tract fibroblasts are divisible into distinct subsets based on their Thy-1 surface expression. Herein, we demonstrate that the expression, induction, and subcellular localization of COX-1 and COX-2 and the downstream PG biosynthesis are markedly different between these subsets. Specifically, Thy-1 + fibroblasts highly express COX-1, which is responsible for high-level PGE 2 production, a feature usually attributed to the COX-2 isoenzyme. In contrast, COX-2, generally considered an inducible isoform, is constitutively expressed in the Thy-1 subset, which only minimally produces PGE 2 . The intracellular signaling pathways for COX regulation also differ between the subsets. Determination of differences in signal transduction, COX expression and localization, and PG production by human reproductive fibroblast subtypes supports the concept of fibroblast heterogeneity and the possibility that these subsets may play unique roles in tissue homeostasis and in inflammation. inflammation; myometrium; lipid mediators; heterogeneity; prostaglandin; cyclooxygenase