Cloning and characterization of extracellular metal protease gene of the algicidal marine bacterium Pseudoalteromonas sp. strain A28

The gene (empI) encoding an extracellular metal protease was isolated from a Pseudoalteromonas sp. strain A28 DNA library. The recombinant EmpI protein was expressed in E. coli and purified. Paper-disk assays showed that the purified protease had potent algicidal activity. A skim milk-polyacrylamide...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2002-06, Vol.66 (6), p.1366-1369
Main Authors: Lee, S.O. (Hiroshima Univ. (Japan)), Kato, J, Nakashima, K, Kuroda, A, Ikeda, T, Takiguchi, N, Ohtake, H
Format: Article
Language:English
Subjects:
1,10-phenanthroline
algicidal marine bacterium
ALGICIDES
BACTERIA
Biological and medical sciences
Biological control and other methods
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Cloning, Molecular
Electrophoresis, Polyacrylamide Gel
empI gene
Endopeptidases - chemistry
Endopeptidases - genetics
Endopeptidases - isolation & purification
Endopeptidases - metabolism
extracellular metal protease
Fundamental and applied biological sciences. Psychology
Metals - metabolism
Miscellaneous
Mission oriented research
MOLECULAR CLONING
Molecular Weight
Parasitic plants. Weeds
Phytopathology. Animal pests. Plant and forest protection
PROTEASES
Pseudoalteromonas
Pseudoalteromonas - enzymology
Pseudoalteromonas - genetics
red tide algae
Restriction Mapping
Weeds
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Description
Summary:The gene (empI) encoding an extracellular metal protease was isolated from a Pseudoalteromonas sp. strain A28 DNA library. The recombinant EmpI protein was expressed in E. coli and purified. Paper-disk assays showed that the purified protease had potent algicidal activity. A skim milk-polyacrylamide gel electrophoresis protease assay showed that the 38-kDa band of protease activity, which co-migrated with purified EmpI and was sensitive to 1,10-phenathroline, was detected in the extracellular supernatant of A28.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.66.1366