Deracemization of α-Methylbenzylamine Using an Enzyme Obtained by In Vitro Evolution

Both the catalytic activity and enantioselectivity of the amine oxidase used in the deracemization of D,L‐α‐methylbenzylamine (see scheme) have been enhanced by in vitro evolution methods to give α‐methylbenzyamine in 77 % yield and 93 % ee.

Saved in:
Bibliographic Details
Published in:Angewandte Chemie (International ed.) 2002-09, Vol.41 (17), p.3177-3180
Main Authors: Alexeeva, Marina, Enright, Alexis, Dawson, Michael J, Mahmoudian, Mahmoud, Turner, Nicholas J
Format: Article
Language:English
Subjects:
Amine Oxidase (Copper-Containing) - chemical synthesis
Amine Oxidase (Copper-Containing) - chemistry
amino acids
Aspergillus niger - enzymology
Chromatography, High Pressure Liquid
Cloning, Molecular
deracemization
Directed Molecular Evolution - methods
Electrophoresis, Polyacrylamide Gel
enzyme catalysis
Escherichia coli - genetics
Escherichia coli - metabolism
Mutagenesis, Site-Directed
Oxidation-Reduction
Peptide Library
Phenethylamines - chemistry
reduction
Stereoisomerism
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Both the catalytic activity and enantioselectivity of the amine oxidase used in the deracemization of D,L‐α‐methylbenzylamine (see scheme) have been enhanced by in vitro evolution methods to give α‐methylbenzyamine in 77 % yield and 93 % ee.
ISSN:1433-7851
1521-3773
DOI:10.1002/1521-3773(20020902)41:17<3177::AID-ANIE3177>3.0.CO;2-P