INHIBITORY EFFECT OF ARTEMISIA CAPILLARIS ON ETHANOL-INDUCED CYTOKINES (TNF-α, IL-1α) SECRETION IN HEP G2 CELLS

• Published in: Immunopharmacology and immunotoxicology 2002-01, Vol.24 (3), p.441-453
• Main Authors: Koo, Hyun-Na, Hong, Seung-Heon, Jeong, Hyun-Ja, Lee, Eun-Hee, Kim, Nam-Guek, Choi, Su-Deock, Ra, Ki-Woong, Kim, Kang-San, Kang, Byung-Ki, Kim, Jeong-Joong, Oh, Jeong Guk, Kim, Hyung-Min
• Format: Article
• Language: English
• Subjects: Apoptosis; Apoptosis - drug effects; Artemisia; Artemisia capillaris; Biological and medical sciences; Cell Cycle - drug effects; Cell Survival - drug effects; Ethanol - toxicity; Ethanol-induced cytotoxicity; General pharmacology; Humans; IL-1α; Interleukin-1 - secretion; Liver - drug effects; Medical sciences; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant Extracts - pharmacology; TNF-α; Tumor Cells, Cultured; Tumor Necrosis Factor-alpha - secretion
• ISSN: 0892-3973; 1532-2513
• DOI: 10.1081/IPH-120014728

ABSTRACT A human hepatoma cell line, Hep G2 cell, is reliable for the study of alcohol-induced hepatotoxicity. In this study, we investigated the effect of an aqueous extract of Artemisia capillaris THUNB (Compositae) plant (AC) on ethanol (EtOH)-induced cytotoxicity in Hep G2 cells. AC (0.5-5 µg mL) inhibited the secretion of EtOH-induced interluekin-1α (IL-1α) and tumor necrosis factor-α (TNF-α). AC also inhibited the EtOH-, IL-1α-, and TNF-α-induced cytotoxicity. Furthermore, we found that AC inhibited the EtOH-induced apoptosis of Hep G2 cells. These results suggest that AC may prevent the EtOH-induced cytotoxicity through inhibition of the apoptosis of Hep G2 cells.