Presence of avipoxvirus DNA in avian dermal squamous cell carcinoma

Dermal squamous cell carcinoma (DSCC; avian keratoacanthoma) is a neoplastic skin lesion of broiler chickens of unknown aetiology. In previous studies, the possibility of the involvement of pox viruses in the cause of DSCC was considered. In this work, a sensitive and specific nested polymerase chai...

Full description

Saved in:
Bibliographic Details
Published in:Avian pathology 2002-06, Vol.31 (3), p.241-246
Main Authors: Fallavena, Luiz C. B., Canal, Cláudio W., Salle, Carlos T. P., Moraes, Hamilton L. S., Rocha, Silvio L. S., Pereira, Rosecler A., Silva, Ari B. da
Format: Article
Language:English
Subjects:
Animals
Avipoxvirus - genetics
Avipoxvirus - isolation & purification
Carcinoma, Squamous Cell - pathology
Carcinoma, Squamous Cell - veterinary
Carcinoma, Squamous Cell - virology
Chick Embryo
Chickens
Deoxyribonucleic acid
Disease
DNA
DNA Primers
DNA, Viral - isolation & purification
Nucleic Acid Amplification Techniques - veterinary
Pathology
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - veterinary
Poultry
Poultry Diseases - pathology
Poultry Diseases - virology
Sensitivity and Specificity
Skin
Skin Neoplasms - pathology
Skin Neoplasms - veterinary
Skin Neoplasms - virology
Specific Pathogen-Free Organisms
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Dermal squamous cell carcinoma (DSCC; avian keratoacanthoma) is a neoplastic skin lesion of broiler chickens of unknown aetiology. In previous studies, the possibility of the involvement of pox viruses in the cause of DSCC was considered. In this work, a sensitive and specific nested polymerase chain reaction (PCR) protocol was developed that could amplify a 419 base pair DNA fragment of fowlpox virus with a detection limit of less than one infectious unit. Fowlpox virus DNA was always detected in skin samples with fowlpox lesions while it was not detected in samples of unrelated diseases such as cowpox, Marek's disease or infectious laryngotracheitis. Some macroscopically normal skin samples from vaccinated and non-vaccinated birds also produced PCR-positive results, corroborating previous studies on the possibility that a latent or chronic form of fowlpox occurs. Fowlpox virus DNA was consistently detected from DSCC skin lesions, and this finding is discussed.
ISSN:0307-9457
1465-3338
DOI:10.1080/03079450220136558