Proinflammatory Cytokines But Not Acute Phase Serum Amyloid A Or C-Reactive Protein, Downregulate Paraoxonase 1 (Pon1) Expression By Hepg2 Cells

The expression of paraoxonase1 (PON1) during inflammation has been investigated in vitro. The alteration of steady state PON1 mRNA in HepG2 cells by interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha), was investigated relative to acute-phase serum amyloid A (A-SAA) mRNA. PON1 m...

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Bibliographic Details
Published in:Amyloid 2002, Vol.9 (3), p.160-164
Main Authors: Kumon, Y., Nakauchi, Y., Suehiro, T., Shiinoki, T., Tanimoto, N., Inoue, M., Nakamura, T, Hashimoto, K., Sipe, J. D.
Format: Article
Language:English
Subjects:
Apolipoproteins - pharmacology
Aryldialkylphosphatase
C-Reactive Protein - metabolism
Carcinoma, Hepatocellular - immunology
Carcinoma, Hepatocellular - metabolism
Carrier Proteins
Cyclic AMP Receptor Protein - pharmacology
Cytokines - immunology
Cytokines - pharmacology
Down-Regulation
Esterases - biosynthesis
Esterases - drug effects
Esterases - genetics
Humans
Serum Amyloid A Protein - metabolism
Serum Amyloid A Protein - pharmacology
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Summary:The expression of paraoxonase1 (PON1) during inflammation has been investigated in vitro. The alteration of steady state PON1 mRNA in HepG2 cells by interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha), was investigated relative to acute-phase serum amyloid A (A-SAA) mRNA. PON1 mRNA expression by HepG2 cells was decreased within three hours of stimulation by IL-1beta or TNF-alpha. Relative to PON1 mRNA expression, the pattern of steady state A-SAA mRNA expression was altered reciprocally and inversely by IL-1beta. These findings suggested that the decrease in serum PON activity after abdominal surgery in our previous clinical study may be ascribed to a decrease in steady state PON1 mRNA expression by liver with proinflammatory cytokines.
ISSN:1350-6129
1744-2818
DOI:10.3109/13506120209114817