Mechanisms underlying increases in SR Ca2+-ATPase activity after exercise in rat skeletal muscle

Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1 Prolonged exercise followed by a brief period of reduced activity has been shown to result in an overshoot in maximal sarcoplasmic reticulum (SR) Ca 2+ -ATPase activity [maximal velocity ( V max )] in rat locomoter...

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Published in:American journal of physiology: endocrinology and metabolism 2003-03, Vol.284 (3), p.E597-E610
Main Authors: Schertzer, J. D, Green, H. J, Duhamel, T. A, Tupling, A. R
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Calcium - metabolism
Calcium-Transporting ATPases - metabolism
Female
Fluorescence
Motor Activity - physiology
Muscle, Skeletal - physiology
Phosphorylation
Rats
Rats, Sprague-Dawley
Sarcoplasmic Reticulum - enzymology
Sarcoplasmic Reticulum Calcium-Transporting ATPases
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Summary:Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1 Prolonged exercise followed by a brief period of reduced activity has been shown to result in an overshoot in maximal sarcoplasmic reticulum (SR) Ca 2+ -ATPase activity [maximal velocity ( V max )] in rat locomoter muscles (Ferrington DA, Reijneveld JC, Bär PR, and Bigelow DJ. Biochim Biophys Acta 1279: 203-213, 1996). To investigate the functional significance and underlying mechanisms for the increase in V max , we analyzed Ca 2+ -ATPase activity and Ca 2+ uptake in SR vesicles from the fast rat gastrocnemius muscles after prolonged running (RUN) and after prolonged running plus 45 min of low-intensity activity (RUN+) or no activity (REC45) and compared them with controls (Con). Although no differences were observed between RUN and Con, both V max and Ca 2+ uptake were higher ( P  
ISSN:0193-1849
1522-1555
DOI:10.1152/ajpendo.00190.2002