Glucose Metabolism of Lactic Acid Bacteria Changed by Quinone-mediated Extracellular Electron Transfer

It can be expected that extracellular electron transfer to regenerate NAD + changes the glucose metabolism of the homofermentative lactic acid bacteria. In this work, the glucose metabolism of Lactobacillus plantarum and Lactococcus lactis was examined in resting cells with 2-amino-3-carboxy-1,4-nap...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2002-10, Vol.66 (10), p.2100-2106
Main Authors: YAMAZAKI, Shin-ichi, KANEKO, Tsutomu, TAKETOMO, Naoki, KANO, Kenji, IKEDA, Tokuji
Format: Article
Language:English
Subjects:
2-amino-3-carboxy-1,4-naphthoquinone
Aerobiosis
Anaerobiosis
Benzoquinones - pharmacology
Biological and medical sciences
Biological Transport
Culture Media
diaphorase
Electron Transport - drug effects
Extracellular Space - drug effects
Extracellular Space - metabolism
Fundamental and applied biological sciences. Psychology
Glucose - metabolism
lactate dehydrogenase
Lactic Acid - metabolism
Lactobacillus - drug effects
Lactobacillus - growth & development
Lactobacillus - metabolism
Lactobacillus plantarum
Lactococcus lactis
Lactococcus lactis - drug effects
Lactococcus lactis - growth & development
Lactococcus lactis - metabolism
NAD - metabolism
Naphthoquinones - pharmacology
Oxidation-Reduction
Propionibacterium - growth & development
Propionibacterium - metabolism
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Summary:It can be expected that extracellular electron transfer to regenerate NAD + changes the glucose metabolism of the homofermentative lactic acid bacteria. In this work, the glucose metabolism of Lactobacillus plantarum and Lactococcus lactis was examined in resting cells with 2-amino-3-carboxy-1,4-naphthoquinone (ACNQ) as the electron transfer mediator and ferricyanide (Fe(CN) 6 3− ) as the extracellular electron acceptor. NADH in the cells was oxidized by ACNQ with the aid of diaphorase, and the reduced ACNQ was reoxidized with Fe(CN) 6 3− . The extracellular electron transfer system promoted the generation of pyruvate, acetate, and acetoin from glucose, and restricted lactate production. Diaphorase activity increased when cultivation was aerobic, and this increased the concentrations of pyruvate, acetate, and acetoin relative to the concentration of lactate to increase in the presence of ACNQ and Fe(CN) 6 3− .
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.66.2100