Effect of intraportal glucagon-like peptide-1 on glucose metabolism in conscious dogs

• Published in: American journal of physiology: endocrinology and metabolism 2003-05, Vol.284 (5), p.E1027-E1036
• Main Authors: Nishizawa, Makoto, Moore, Mary Courtney, Shiota, Masakazu, Gustavson, Stephanie M, Snead, Wanda L, Neal, Doss W, Cherrington, Alan D
• Format: Article
• Language: English
• Subjects: Alanine - blood; Animals; Blood Glucose - analysis; Cardiovascular System - drug effects; Dogs; Fatty Acids, Nonesterified - blood; Glucagon - administration & dosage; Glucagon-Like Peptide 1; Glucose - metabolism; Glycerol - blood; Glycogen - biosynthesis; Hormones - blood; Infusions, Intravenous; Lactic Acid - blood; Liver - metabolism; Liver Circulation - drug effects; Osmolar Concentration; Peptide Fragments - administration & dosage; Portal Vein; Protein Precursors - administration & dosage
• ISSN: 0193-1849; 1522-1555
• DOI: 10.1152/ajpendo.00503.2002

Department of Molecular Physiology & Biophysics and Diabetes Research and Training Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 Arteriovenous difference and tracer ([3- 3 H]glucose) techniques were used in 42-h-fasted conscious dogs to identify any insulin-like effects of intraportally administered glucagon-like peptide 1-(7-36)amide (GLP-1). Each study consisted of an equilibration, a basal, and three 90-min test periods (P1, P2, and P3) during which somatostatin, intraportal insulin (3-fold basal) and glucagon (basal), and peripheral glucose were infused. Saline was infused intraportally in P1. During P2 and P3, GLP-1 was infused intraportally at 0.9 and 5.1   pmol · kg 1 · min 1 in eight dogs, at 10 and 20 pmol · kg 1 · min 1 in seven dogs, and at 0 pmol · kg 1 · min 1 in eight dogs (control group). Net hepatic glucose uptake was significantly enhanced during GLP-1 infusion at 20 pmol · kg 1 · min 1 [21.8 vs. 13.4 µmol · kg 1 · min 1 (control), P