Effects of added dietary taurine on erythrocyte lipids and oxidative stress in rabbits fed a high cholesterol diet

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2002-12, Vol.66 (12), p.2701-2705
• Main Authors: Balkan, J. (University of Istanbul (Turkey)), Oeztezcan, S, Aykac-Toker, G, Uysal, M
• Format: Article
• Language: English
• Subjects: Anemia, Hemolytic - chemically induced; Animals; Biological and medical sciences; CHOLESTEROL; Cholesterol - metabolism; Cholesterol, Dietary - adverse effects; Cholesterol, Dietary - metabolism; DIET; Dietary Supplements; erythrocyte; ERYTHROCYTES; Erythrocytes - chemistry; Erythrocytes - drug effects; FEED ADDITIVES; Fundamental and applied biological sciences. Psychology; Hemolysis - drug effects; high cholesterol diet; Hydrogen Peroxide - pharmacology; LIPID PEROXIDATION; Lipid Peroxidation - drug effects; LIPIDS; Malondialdehyde - metabolism; Osmolar Concentration; Oxidative Stress - drug effects; rabbit; RABBITS; STRESS; TAURINE; Taurine - administration & dosage; Taurine - pharmacology; Triglycerides - blood; Vertebrates: blood, hematopoietic organs, reticuloendothelial system
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.66.2701

Lipid peroxidation leads to damage of polyunsaturated fatty acids of membrane phospholipids. The contribution of oxidative stress to hypercholesterolemia-induced hemolytic anemia and the effects of addition of taurine on erythrocyte lipid composition, oxidative stres, and hematological data were studied in rabbits fed on a high cholesterol (HC) diet (1%, w/w) for 2 months. The effects of taurine on erythrocyte hemolysis and H 2 O 2 -induced lipid peroxidation were investigated in normal rabbit erythrocytes in vitro. The HC diet resulted in increases in plasma lipids and lipid peroxide levels as well as increases in cholesterol levels and the cholesterol:phospholipid ratio in the erythrocytes. This diet caused a hemolytic anemia, but lipid peroxide levels remained unchanged in the erythrocytes of the rabbits. Taurine (2.5%, w/w) added to the food has an ameliorating effect on plasma lipids and lipid peroxide levels in rabbits fed on a HC diet. This treatment also caused decreases in elevated erythrocyte cholesterol levels and cholesterol:phospholipid ratio due to the HC diet, but it did not prevent the hemolytic anemia and did not change erythrocyte lipid peroxide levels. In addition, in an in vitro study, taurine did not protect erythrocytes against H 2 O 2 -induced hemolysis or lipid peroxidation. These results show that the HC diet causes hemolytic anemia without any changes in erythrocyte lipid peroxidation, and taurine treatment was not effective against hemolytic anemia caused by the HC diet.