Role of NF-kappaB and AP-1 on Helicobater pylori-induced IL-8 expression in AGS cells

Oxygen radicals are important regulators in Helicobacter pylori-induced gastric ulceration and carcinogenesis. IL-8 may be regulated by oxidant-sensitive transcription factors, NF-kappaB, and AP-1. The present study aims to investigate whether H. pylori-induced IL-8 expression is regulated by NF-kap...

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Published in:Digestive diseases and sciences 2003-02, Vol.48 (2), p.257
Main Authors: Chu, Sang Hui, Kim, Hyeyoung, Seo, Jeong Yeon, Lim, Joo Weon, Mukaida, Naofumi, Kim, Kyung Hwan
Format: Article
Language:English
Subjects:
Analysis of Variance
Base Sequence
Blotting, Northern
Blotting, Western
Cells, Cultured
Epithelial Cells
Gastric Mucosa - cytology
Gastric Mucosa - microbiology
Helicobacter Infections - pathology
Helicobacter pylori
Humans
Interleukin-8 - analysis
Interleukin-8 - metabolism
Molecular Sequence Data
NF-kappa B - analysis
NF-kappa B - metabolism
Polymerase Chain Reaction
Probability
Reactive Oxygen Species
RNA, Messenger - analysis
Sensitivity and Specificity
Stomach Ulcer - microbiology
Stomach Ulcer - pathology
Transcription Factor AP-1 - analysis
Transcription Factor AP-1 - metabolism
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Summary:Oxygen radicals are important regulators in Helicobacter pylori-induced gastric ulceration and carcinogenesis. IL-8 may be regulated by oxidant-sensitive transcription factors, NF-kappaB, and AP-1. The present study aims to investigate whether H. pylori-induced IL-8 expression is regulated by NF-kappaB and AP-1 in gastric epithelial AGS cells and whether this transcriptional regulation of IL-8 is inhibited by N-acetylcysteine (NAC). As a result, H. pylori induced the expression of mRNA and protein for IL-8 via activation of NF-kappaB and AP-1. NF-kappaB activation accompanied by a decrease in I-kappaBalpha and activated AP-1 complex was a c-jun/c-fos heterodimer in H. pylori-infected AGS cells. NAC inhibited H. pylori-induced activation of transcription factors and IL-8 expression in AGS cells. In conclusion, oxygen radicals induce the activation of NF-kappaB and AP-1 and IL-8 expression. Antioxidants such as NAC might be useful anti-inflammatory agents by inhibiting activation of transcription factors and decreasing IL-8 production in H. pylori-induced gastric inflammation.
ISSN:0163-2116