Chemoprevention of Benzo(a)pyrene-induced Lung Tumors in Mice by the Farnesyltransferase Inhibitor R115777

• Published in: Clinical cancer research 2003-05, Vol.9 (5), p.1927-1930
• Main Authors: GUNNING, William T, KRAMER, Paula M, LUBET, Ronald A, STEELE, Vernon E, END, David W, WOUTERS, Walter, PEREIRA, Michael A
• Format: Article
• Language: English
• Subjects: Alkyl and Aryl Transferases - antagonists & inhibitors; Animals; Antineoplastic Agents - therapeutic use; Benzo(a)pyrene - toxicity; Biological and medical sciences; Carcinogenesis, carcinogens and anticarcinogens; Chemical agents; Chemoprevention; Enzyme Inhibitors - therapeutic use; Female; Lung Neoplasms - chemically induced; Lung Neoplasms - prevention & control; Medical sciences; Mice; Mice, Inbred A; Quinolones - therapeutic use; Tumors
• ISSN: 1078-0432; 1557-3265

Purpose: Inhibitors of farnesyltransferase ( e.g., R115777) are being developed for therapy and prevention of various cancers. The efficacy of R115777 [Zarnestra; ( B )-6-[amino(4-chlorophenyl)(1-methyl-1 H -imidazol-5-yl)-methyl]-4-(3-chlorophenyl)-1-methyl-2(1 H )-quinolinone] to prevent the development of lung tumors in mice was determined. Experimental Design: Female strain A mice (7–8 weeks of age) were given 100 mg/kg benzo( a )pyrene [B(a)P] by i.p. injection, and 4 or 14 weeks later, they were given 50 or 100 mg/kg R115777 by oral gavage 5 days/week. The mice were sacrificed 22 weeks after they received the B(a)P. Results : Tumor multiplicity was 5.0 ± 0.85, 4.5 ± 0.52, 2.1 ± 0.31, and 1.5 ± 0.31 tumors/mouse in mice that received 0, 50, 100 (weeks 4–22), or 100 (weeks 14–22) mg/kg R115777. Thus, 100 mg/kg R115777 was similarly effective in preventing lung tumors when administered during the promotional phase of carcinogenesis [that is, either 4 or 14 weeks after B(a)P], whereas the lower dose of 50 mg/kg R115777 was ineffective. The proliferating cell nuclear antigen labeling index was also significantly reduced in lung tumors from mice treated with 100 mg/kg R115777 starting at 4 or 14 weeks. Conclusions: These results demonstrated that R115777 can prevent the development of lung tumors in the A/J mouse model, where tumors routinely have mutations in the Ki- Ras oncogene.