Expression of GPI-80, a beta2-integrin-associated glycosylphosphatidylinositol-anchored protein, requires neutrophil differentiation with dimethyl sulfoxide in HL-60 cells

• Published in: Experimental cell research 2003-06, Vol.286 (2), p.199
• Main Authors: Takeda, Yuji, Fu, Junfen, Suzuki, Kichiya, Sendo, Dai, Nitto, Takeaki, Sendo, Fujiro, Araki, Yoshihiko
• Format: Article
• Language: English
• Subjects: Amidohydrolases; Antibodies - pharmacology; CD11 Antigens - metabolism; CD18 Antigens - genetics; CD18 Antigens - metabolism; Cell Adhesion - drug effects; Cell Adhesion - physiology; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - metabolism; Cell Differentiation - drug effects; Cell Differentiation - physiology; Chemotaxis, Leukocyte - drug effects; Chemotaxis, Leukocyte - genetics; Cholecalciferol - pharmacology; Dimethyl Sulfoxide - pharmacology; Gene Expression Regulation - drug effects; Gene Expression Regulation - physiology; GPI-Linked Proteins; Granulocyte Colony-Stimulating Factor - pharmacology; Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology; HL-60 Cells; Humans; Hydrolases; Models, Biological; Myeloid Cells - drug effects; Myeloid Cells - metabolism; Neutrophils - drug effects; Neutrophils - metabolism; Receptors, Transferrin - metabolism; Tetradecanoylphorbol Acetate - pharmacology; Tretinoin - pharmacology
• ISSN: 0014-4827

GPI-80 is a member of the amidohydrolase family that has been proposed as a potential regulator of beta2-integrin-dependent leukocyte adhesion. GPI-80 is expressed mainly in human neutrophils. Our previous studies suggested that GPI-80 expression might be associated with myeloid differentiation. To verify this, we examined whether GPI-80 is expressed on the human promyelocytic leukemia cell line HL-60 following treatment with differentiation inducers. GPI-80 expression was induced in cells treated with dimethyl sulfoxide (DMSO) to stimulate differentiation down the neutrophil pathway. On the other hand, all-trans-retinoic acid (ATRA), another neutrophil-inducing reagent, induced no clear GPI-80 expression. Potent monocyte-inducing reagents such as 1alpha,25-dihydroxyvitamin D(3) or phorbol 12-myristate 13-acetate also had no significant effect on the protein expression. GPI-80-positive cells were found in the well-differentiated CD11b-positive and transferrin-receptor-negative cell population. Granulocyte colony-stimulating factor, which augments neutrophil differentiation of HL-60 cells, up-regulated GPI-80 expression in the presence of DMSO. Granulocyte/macrophage colony-stimulating factor, which is known to suppress the neutrophil maturation of cells, inhibited expression. Adhesion of DMSO-induced cells was regulated by anti-GPI-80 monoclonal antibody, similar to the regulation observed in neutrophils. These results suggest that use of DMSO to induce neutrophil differentiation provides suitable conditions for GPI-80 expression, and that this culture system may be a helpful model for further study of the regulation of GPI-80 expression during myeloid differentiation.