Cloning and Prokaryotic Expression of a cDNA Encoding a Putative Mitochondrial Malate Dehydrogenase in Oryza sativa

Malate dehydrogenase (MDH) has been characterized as a key player in oxaloacetate (OAA) biosynthesis mechanism in citrate acid cycle that generates reducing powers for further assimilation in the whole cell. Here we present the cloning, characterization and prokaryotic expression of a putative Mdh (...

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Bibliographic Details
Published in:DNA sequence 2003-01, Vol.14 (1), p.75-77
Main Authors: Lin, Chang-Fa, Jiang, Li-Zhi, Zhang, Xiao-Ning, Qian, Xiao-Yin, Liang, Zong-Suo, Yang, Jin-Shui
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA, Complementary - chemistry
DNA, Complementary - genetics
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Escherichia coli - genetics
Eucalyptus
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Malate Dehydrogenase - genetics
Malate Dehydrogenase - metabolism
Mdh gene
Mitochondria - enzymology
Mitochondrial Malate Dehydrogenase
Molecular Sequence Data
Oryza - enzymology
Oryza - genetics
Oryza Sativa
Prokaryotic Expression
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Tissue-specific Expression
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Summary:Malate dehydrogenase (MDH) has been characterized as a key player in oxaloacetate (OAA) biosynthesis mechanism in citrate acid cycle that generates reducing powers for further assimilation in the whole cell. Here we present the cloning, characterization and prokaryotic expression of a putative Mdh ( OsmMDH ) in Oryza sativa. Sequence alignment shows that there is a high homology between the deduced amino acid sequence of OsmMDH and MDH portein in Eucalyptus gunnii (80%), as well as between the deduced amino acid sequence of OsmMDH and other MDHs. Moreover, pI and the mitochondrial location of OsmMDH are predicted. The tissue-specific expression pattern of OsmMDH reveals that it is abundant in young panicle and immature seed, while its expression level is mush lower in leaf and root. Its expression in E. coli BL21 as a fusion gene is studied further.
ISSN:1042-5179
1029-2365
DOI:10.1080/1042517031000062800