Packaging cells for avian leukosis virus-based vectors with various host ranges

Using our previously described Haydee semipackaging cell line (F. L. Cosset, C. Legras, Y. Chebloune, P. Savatier, P. Thoraval, J. L. Thomas, J. Samarut, V. M. Nigon, and G. Verdier, J. Virol. 64:1070-1078, 1990) which produces avian leukosis virus gag and pol proteins, we have constructed packaging...

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Bibliographic Details
Published in:Journal of Virology 1992-09, Vol.66 (9), p.5671-5676
Main Authors: Cosset, F.L. (INRA, CNRS, Villeurbanne, France), Ronfort, C, Molina, R.M, Flamant, F, Drynda, A, Benchaibi, M, Valsesia, S, Nigon, V.M, Verdier, G
Format: Article
Language:English
Subjects:
Animals
Avian Leukosis Virus - genetics
Avian Leukosis Virus - growth & development
Biological and medical sciences
Birds - microbiology
Cell Line
CULTIVO DE CELULAS
CULTURE DE CELLULE
Fundamental and applied biological sciences. Psychology
Genetic Vectors - genetics
Genetics
Helper Viruses
HOTE
HUESPEDES
Life Sciences
Microbiology
Microbiology and Parasitology
ONCOVIRUS AVIAIRE
ONCOVIRUS AVIAR
Plasmids - genetics
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - genetics
Species Specificity
Transfection
VECTEUR DE MALADIE
VECTORES
Virology
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Summary:Using our previously described Haydee semipackaging cell line (F. L. Cosset, C. Legras, Y. Chebloune, P. Savatier, P. Thoraval, J. L. Thomas, J. Samarut, V. M. Nigon, and G. Verdier, J. Virol. 64:1070-1078, 1990) which produces avian leukosis virus gag and pol proteins, we have constructed packaging cells with subgroups B, C, and E envelope specificities. This allows us to produce helper-free avian leukosis virus particles carrying the lacZ reporter gene and the A, B, C, or E subgroup specificities. Titers of the recombinant lacZ virus are shown to be dependent upon the type of the env subgroup and the target avian cell
ISSN:0022-538X
1098-5514
DOI:10.1128/JVI.66.9.5671-5676.1992