The Analysis of Mitoses in Single Living Cells by Interference Microscopy

A new method of using the interference microscope is described which enables a continuous record to be obtained of changes in dry mass and concentration during the growth of a single tissue cell. The method has been applied to the analysis of changes of dry mass in the chromosomes, spindle and cell-...

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Bibliographic Details
Published in:Proceedings of the Royal Society of London. Series B, Biological sciences Biological sciences, 1961-01, Vol.153 (952), p.357-366
Main Authors: Ambrose, E. J., Bajer, A
Format: Article
Language:English
Subjects:
Anaphase
Birefringence
Cell Division
Chromosomes
Clear zones
Cytoplasm
Diploidy
Endosperm
Humans
Metaphase
Microscopes
Microscopy, Interference
Mitosis
Old Medline
Prophase
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Summary:A new method of using the interference microscope is described which enables a continuous record to be obtained of changes in dry mass and concentration during the growth of a single tissue cell. The method has been applied to the analysis of changes of dry mass in the chromosomes, spindle and cell-plate area during mitosis in endosperm. The results of these observations have been compared with earlier studies of birefringence during mitosis. It is shown that birefringence develops under conditions of progressively increasing concentration of macro-molecular material.
ISSN:0962-8452
0080-4649
0950-1193
1471-2954
2053-9193
DOI:10.1098/rspb.1961.0006