6-Aminopenicillanic Acid. III. Isolation and Purification

Methods are described for the isolation of pure crystalline 6-aminopenicillanic acid from penicillin fermentation broth. With low potency fermentation broth it was found to be con­venient to prepare a concentrate of the clarified broth before proceeding with other extraction methods. Negligible amou...

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Bibliographic Details
Published in:Proceedings of the Royal Society of London. Series B, Biological sciences Biological sciences, 1961-08, Vol.154 (957), p.498-508
Main Authors: Batchelor, F. R., Chain, Ernst Boris, Hardy, T. L., Mansford, K. R. L., Rolinson, G. N.
Format: Article
Language:English
Subjects:
Adsorption
Charcoal
Elution
Fermentation
Hydroxides
Old Medline
Paper chromatography
Penicillanic Acid
Penicillin
Penicillins - chemistry
Resins
Sodium
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Summary:Methods are described for the isolation of pure crystalline 6-aminopenicillanic acid from penicillin fermentation broth. With low potency fermentation broth it was found to be con­venient to prepare a concentrate of the clarified broth before proceeding with other extraction methods. Negligible amounts of 6-aminopenicillanic acid could be extracted directly into organic solvents such as butanol or butyl acetate. Partial purification could be achieved by adsorption and elution from alumina and charcoal. Adsorption and elution from a suitable ion exchange resin proved more effective. Crystalline 6-aminopenicillanic acid was then obtained by concentration of this ion exchange eluate and pH adjustment to the point of minimum solubility. Alternatively, further purification of the ion exchange eluate could be achieved by chromatography on a column of cellulose before proceeding with the crystal­lization step. Some properties of the isolated 6-aminopenicillanic acid are also given.
ISSN:0962-8452
0080-4649
0950-1193
1471-2954
2053-9193
DOI:10.1098/rspb.1961.0047