The effect of intra-abdominal pressure on the generation of 8-iso prostaglandin F2alpha during laparoscopy in rabbits

Carbon dioxide pneumoperitoneum induces peritoneal oxidative stress. The aim of this study was to verify the effect of intra-abdominal pressure on oxidative stress in the peritoneum and on post-operative adhesion formation. Forty-one rabbits underwent laparoscopic surgery: either gasless, or with CO...

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Bibliographic Details
Published in:Human reproduction (Oxford) 2003-10, Vol.18 (10), p.2181
Main Authors: de Souza, Angela M Bentes, Wang, Chi Chiu, Chu, Ching Yan, Lam, Po Mui, Rogers, Michael Scott
Format: Article
Language:English
Subjects:
Abdominal Cavity - physiology
Animals
Carbon Dioxide - adverse effects
Dinoprost - analogs & derivatives
Dinoprost - biosynthesis
Female
Laparoscopy
Peritoneal Diseases - etiology
Pneumoperitoneum, Artificial - adverse effects
Postoperative Period
Pressure
Rabbits
Time Factors
Tissue Adhesions - etiology
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Summary:Carbon dioxide pneumoperitoneum induces peritoneal oxidative stress. The aim of this study was to verify the effect of intra-abdominal pressure on oxidative stress in the peritoneum and on post-operative adhesion formation. Forty-one rabbits underwent laparoscopic surgery: either gasless, or with CO(2)-pneumoperitoneum at pressures of 5, 10 or 15 mmHg. Serial parietal peritoneal biopsies were taken at various time-points: immediately after reaching the abdominal cavity, 30, 60, 90 and 120 min afterwards, and 15 min after abdominal desufflation. 8-iso prostaglandin F(2alpha) (8-iso PGF(2alpha)), a marker of oxidative stresss, was assayed by enzyme immunoassay and adhesion formation was scored by second-look laparoscopy on day 14. The gasless group showed no significant changes in 8-iso PGF(2alpha). Conversely, significant changes occurred in CO(2)-pneumoperitoneum in a time- and pressure-dependent manner. Adhesions developed only in the CO(2)-pneumoperitoneum groups, and total adhesion score was correlated with the amount of CO(2) insufflated and intra-abdominal pressure, but not with 8-iso PGF(2alpha), which was correlated with intra-abdominal pressure. Intra-abdominal pressure increased 8-iso PGF(2alpha) in the parietal peritoneum in a graded fashion, whilst gasless laparoscopy had no impact. It also influenced the frequency and severity of adhesion formation, but no causal link was found between 8-iso PGF(2alpha) and post-operative adhesion formation.
ISSN:0268-1161