The effect of intra-abdominal pressure on the generation of 8-iso prostaglandin F2alpha during laparoscopy in rabbits

• Published in: Human reproduction (Oxford) 2003-10, Vol.18 (10), p.2181
• Main Authors: de Souza, Angela M Bentes, Wang, Chi Chiu, Chu, Ching Yan, Lam, Po Mui, Rogers, Michael Scott
• Format: Article
• Language: English
• Subjects: Abdominal Cavity - physiology; Animals; Carbon Dioxide - adverse effects; Dinoprost - analogs & derivatives; Dinoprost - biosynthesis; Female; Laparoscopy; Peritoneal Diseases - etiology; Pneumoperitoneum, Artificial - adverse effects; Postoperative Period; Pressure; Rabbits; Time Factors; Tissue Adhesions - etiology
• ISSN: 0268-1161

Carbon dioxide pneumoperitoneum induces peritoneal oxidative stress. The aim of this study was to verify the effect of intra-abdominal pressure on oxidative stress in the peritoneum and on post-operative adhesion formation. Forty-one rabbits underwent laparoscopic surgery: either gasless, or with CO(2)-pneumoperitoneum at pressures of 5, 10 or 15 mmHg. Serial parietal peritoneal biopsies were taken at various time-points: immediately after reaching the abdominal cavity, 30, 60, 90 and 120 min afterwards, and 15 min after abdominal desufflation. 8-iso prostaglandin F(2alpha) (8-iso PGF(2alpha)), a marker of oxidative stresss, was assayed by enzyme immunoassay and adhesion formation was scored by second-look laparoscopy on day 14. The gasless group showed no significant changes in 8-iso PGF(2alpha). Conversely, significant changes occurred in CO(2)-pneumoperitoneum in a time- and pressure-dependent manner. Adhesions developed only in the CO(2)-pneumoperitoneum groups, and total adhesion score was correlated with the amount of CO(2) insufflated and intra-abdominal pressure, but not with 8-iso PGF(2alpha), which was correlated with intra-abdominal pressure. Intra-abdominal pressure increased 8-iso PGF(2alpha) in the parietal peritoneum in a graded fashion, whilst gasless laparoscopy had no impact. It also influenced the frequency and severity of adhesion formation, but no causal link was found between 8-iso PGF(2alpha) and post-operative adhesion formation.