Inhibitory effect of kaurane type diterpenoids from Acanthopanax koreanum on TNF-alpha secretion from trypsin-stimulated HMC-1 cells

Five known kaurane type diterpenoids, 16alphaH,17-isovaleryloxy-ent-kauran-19-oic acid (1), 16alpha-hydroxy-17-isovaleryloxy-ent-kauran-19-oic acid (2), paniculoside-IV (3), 16alpha-hydroxy-ent-kauran-19-oic acid (4), and ent-kaur-16-en-19-oic acid (5) were isolated from the root of Acanthopanax kor...

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Bibliographic Details
Published in:Archives of pharmacal research 2003-09, Vol.26 (9), p.731
Main Authors: Cai, Xing Fu, Shen, Guanghai, Dat, Nguyen Tien, Kang, Ok Hwa, Lee, Young Mi, Lee, Jung Joon, Kim, Young Ho
Format: Article
Language:English
Subjects:
Diterpenes, Kaurane - chemistry
Diterpenes, Kaurane - isolation & purification
Diterpenes, Kaurane - pharmacology
Eleutherococcus - chemistry
Flavonoids - pharmacology
Humans
Korea
Leukemia, Mast-Cell - chemically induced
Leukemia, Mast-Cell - metabolism
Luteolin
Methanol
Plant Extracts - chemistry
Plant Roots - chemistry
Plants, Medicinal - chemistry
Trypsin - pharmacology
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha - antagonists & inhibitors
Tumor Necrosis Factor-alpha - metabolism
Tumor Necrosis Factor-alpha - secretion
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Summary:Five known kaurane type diterpenoids, 16alphaH,17-isovaleryloxy-ent-kauran-19-oic acid (1), 16alpha-hydroxy-17-isovaleryloxy-ent-kauran-19-oic acid (2), paniculoside-IV (3), 16alpha-hydroxy-ent-kauran-19-oic acid (4), and ent-kaur-16-en-19-oic acid (5) were isolated from the root of Acanthopanax koreanum by repeated column chromatography and reversed phase preparative HPLC. The structures of these compounds were established from physicochemical and spectral data. Among the isolated compounds 16alphaH,17-isovaleryloxy-ent-kauran-19-oic acid (1) showed potent inhibitory activity (IC50 value, 16.2 uM) on TNF-alpha secretion from HMC-1, a trypsin-stimulated human leukemic mast cell line.
ISSN:0253-6269
DOI:10.1007/bf02976683